En face Doppler total retinal blood flow measurement with 70 kHz spectral optical coherence tomography

Ou Tan, Gangjun Liu, Liu Liang, Simon S. Gao, Alex D. Pechauer, Jia Yali, David Huang

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

An automated algorithm was developed for total retinal blood flow (TRBF) using 70-kHz spectral optical coherence tomography (OCT). The OCT was calibrated for the transformation from Doppler shift to speed based on a flow phantom. The TRBF scan pattern contained five repeated volume scans (2 × 2 mm) obtained in 3 s and centered on central retinal vessels in the optic disc. The TRBF was calculated using an en face Doppler technique. For each retinal vein, blood flow was measured at an optimal plane where the calculated flow was maximized. The TRBF was calculated by summing flow in all veins. The algorithm tracked vascular branching so that either root or branch veins are summed, but never both. The TRBF in five repeated volumes were averaged to reduce variation due to cardiac cycle pulsation. Finally, the TRBF was corrected for eye length variation. Twelve healthy eyes and 12 glaucomatous eyes were enrolled to test the algorithm. The TRBF was 45.4 ± 6.7 μl/min for healthy control and 34.7 ± 7.6 μl/min for glaucomatous participants (p-value = 0.01). The intravisit repeatability was 8.6% for healthy controls and 8.4% for glaucoma participants. The proposed automated method provided repeatable TRBF measurement.

Original languageEnglish (US)
Article number066004
JournalJournal of Biomedical Optics
Volume20
Issue number6
DOIs
StatePublished - Jun 1 2015

Fingerprint

Optical tomography
flow measurement
Flow measurement
blood flow
Blood
tomography
veins
glaucoma
Doppler effect
vessels
Optics
optics
cycles
shift

Keywords

  • Doppler optical coherence tomography
  • en face Doppler
  • multiple plane
  • total retinal blood flow

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

En face Doppler total retinal blood flow measurement with 70 kHz spectral optical coherence tomography. / Tan, Ou; Liu, Gangjun; Liang, Liu; Gao, Simon S.; Pechauer, Alex D.; Yali, Jia; Huang, David.

In: Journal of Biomedical Optics, Vol. 20, No. 6, 066004, 01.06.2015.

Research output: Contribution to journalArticle

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