Eimeria tenella

Quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites

M. S. Crane, Douglas Norman, M. J. Gnozzio, A. C. Tate, M. Gammon, P. K. Murray

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Murine, polyclonal and monoclonal antibodies, raised against sporozoites of Eimeria tenella, were tested for their ability to neutralize sporozoite infectivity in vitro and in vivo. Neutralization was effected via three mechanisms. Firstly, sporozoites fixed complement, at low titres, and lysis occurred by the alternative poathway of complement activation. Secondly, in the absence of complement activity, the murine heat-inactivated, hyperimmune antiserum neutralized sporozoites at relatively low titres. At high titres, even though sporozoites were agglutinated, neither the heat-inactivated hyperimmune antiserum nor the monoclonal antibody neutralized sporozoites. Finally, in the presence of complement and specific antibodies, at titres which by themselves would not neutralize sporozoites, neutralization was effected due to lysis via the classical pathway of complement activation.

Original languageEnglish (US)
Pages (from-to)467-480
Number of pages14
JournalParasite Immunology
Volume8
Issue number5
StatePublished - 1986
Externally publishedYes

Fingerprint

Eimeria tenella
Sporozoites
Monoclonal Antibodies
Immune Sera
Hot Temperature
Classical Complement Pathway
Complement Activation
In Vitro Techniques
Antibodies

ASJC Scopus subject areas

  • Parasitology
  • Immunology

Cite this

Eimeria tenella : Quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites. / Crane, M. S.; Norman, Douglas; Gnozzio, M. J.; Tate, A. C.; Gammon, M.; Murray, P. K.

In: Parasite Immunology, Vol. 8, No. 5, 1986, p. 467-480.

Research output: Contribution to journalArticle

Crane, M. S. ; Norman, Douglas ; Gnozzio, M. J. ; Tate, A. C. ; Gammon, M. ; Murray, P. K. / Eimeria tenella : Quantitative in vitro and in vivo studies on the effects of mouse polyclonal and monoclonal antibodies on sporozoites. In: Parasite Immunology. 1986 ; Vol. 8, No. 5. pp. 467-480.
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