Efferent projections of neuropeptide Y-expressing neurons of the dorsomedial hypothalamus in chronic hyperphagic models

Shin J. Lee, Melissa Kirigiti, Sarah R. Lindsley, Alberto Loche, Christopher (Chris) Madden, Shaun Morrison, M (Susan) Smith, Kevin Grove

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

The dorsomedial hypothalamus (DMH) has long been implicated in feeding behavior and thermogenesis. The DMH contains orexigenic neuropeptide Y (NPY) neurons, but the role of these neurons in the control of energy homeostasis is not well understood. NPY expression in the DMH is low under normal conditions in adult rodents but is significantly increased during chronic hyperphagic conditions such as lactation and diet-induced obesity (DIO). To understand better the role of DMH-NPY neurons, we characterized the efferent projections of DMH-NPY neurons using the anterograde tracer biotinylated dextran amine (BDA) in lactating rats and DIO mice. In both models, BDA- and NPY-colabeled fibers were limited mainly to the hypothalamus, including the paraventricular nucleus of the hypothalamus (PVH), lateral hypothalamus/perifornical area (LH/PFA), and anteroventral periventricular nucleus (AVPV). Specifically in lactating rats, BDA-and NPY-colabeled axonal swellings were in close apposition to cocaine- and amphetamine-regulated transcript (CART)-expressing neurons in the PVH and AVPV. Although the DMH neurons project to the rostral raphe pallidus (rRPa), these projections did not contain NPY immunoreactivity in either the lactating rat or the DIO mouse. Instead, the majority of BDA-labeled fibers in the rRPa were orexin positive. Furthermore, DMH-NPY projections were not observed within the nucleus of the solitary tract (NTS), another brainstem site critical for the regulation of sympathetic outflow. The present data suggest that NPY expression in the DMH during chronic hyperphagic conditions plays important roles in feeding behavior and thermogenesis by modulating neuronal functions within the hypothalamus, but not in the brainstem. J. Comp. Neurol. 521:1891-1914, 2013.

Original languageEnglish (US)
Pages (from-to)1891-1914
Number of pages24
JournalJournal of Comparative Neurology
Volume521
Issue number8
DOIs
StatePublished - Jun 1 2013

Fingerprint

Neuropeptide Y
Hypothalamus
Neurons
Anterior Hypothalamus
Thermogenesis
Obesity
Paraventricular Hypothalamic Nucleus
Feeding Behavior
Diet
Brain Stem
Efferent Neurons
Lateral Hypothalamic Area
Solitary Nucleus
Amphetamine
Cocaine
Lactation
Rodentia
Homeostasis

Keywords

  • Food intake
  • Hypothalamus
  • Lactation
  • Obesity
  • Thermogenesis

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Efferent projections of neuropeptide Y-expressing neurons of the dorsomedial hypothalamus in chronic hyperphagic models. / Lee, Shin J.; Kirigiti, Melissa; Lindsley, Sarah R.; Loche, Alberto; Madden, Christopher (Chris); Morrison, Shaun; Smith, M (Susan); Grove, Kevin.

In: Journal of Comparative Neurology, Vol. 521, No. 8, 01.06.2013, p. 1891-1914.

Research output: Contribution to journalArticle

Lee, Shin J. ; Kirigiti, Melissa ; Lindsley, Sarah R. ; Loche, Alberto ; Madden, Christopher (Chris) ; Morrison, Shaun ; Smith, M (Susan) ; Grove, Kevin. / Efferent projections of neuropeptide Y-expressing neurons of the dorsomedial hypothalamus in chronic hyperphagic models. In: Journal of Comparative Neurology. 2013 ; Vol. 521, No. 8. pp. 1891-1914.
@article{d265db0294c94a4d967b44366ffa03e9,
title = "Efferent projections of neuropeptide Y-expressing neurons of the dorsomedial hypothalamus in chronic hyperphagic models",
abstract = "The dorsomedial hypothalamus (DMH) has long been implicated in feeding behavior and thermogenesis. The DMH contains orexigenic neuropeptide Y (NPY) neurons, but the role of these neurons in the control of energy homeostasis is not well understood. NPY expression in the DMH is low under normal conditions in adult rodents but is significantly increased during chronic hyperphagic conditions such as lactation and diet-induced obesity (DIO). To understand better the role of DMH-NPY neurons, we characterized the efferent projections of DMH-NPY neurons using the anterograde tracer biotinylated dextran amine (BDA) in lactating rats and DIO mice. In both models, BDA- and NPY-colabeled fibers were limited mainly to the hypothalamus, including the paraventricular nucleus of the hypothalamus (PVH), lateral hypothalamus/perifornical area (LH/PFA), and anteroventral periventricular nucleus (AVPV). Specifically in lactating rats, BDA-and NPY-colabeled axonal swellings were in close apposition to cocaine- and amphetamine-regulated transcript (CART)-expressing neurons in the PVH and AVPV. Although the DMH neurons project to the rostral raphe pallidus (rRPa), these projections did not contain NPY immunoreactivity in either the lactating rat or the DIO mouse. Instead, the majority of BDA-labeled fibers in the rRPa were orexin positive. Furthermore, DMH-NPY projections were not observed within the nucleus of the solitary tract (NTS), another brainstem site critical for the regulation of sympathetic outflow. The present data suggest that NPY expression in the DMH during chronic hyperphagic conditions plays important roles in feeding behavior and thermogenesis by modulating neuronal functions within the hypothalamus, but not in the brainstem. J. Comp. Neurol. 521:1891-1914, 2013.",
keywords = "Food intake, Hypothalamus, Lactation, Obesity, Thermogenesis",
author = "Lee, {Shin J.} and Melissa Kirigiti and Lindsley, {Sarah R.} and Alberto Loche and Madden, {Christopher (Chris)} and Shaun Morrison and Smith, {M (Susan)} and Kevin Grove",
year = "2013",
month = "6",
day = "1",
doi = "10.1002/cne.23265",
language = "English (US)",
volume = "521",
pages = "1891--1914",
journal = "Journal of Comparative Neurology",
issn = "0021-9967",
publisher = "Wiley-Liss Inc.",
number = "8",

}

TY - JOUR

T1 - Efferent projections of neuropeptide Y-expressing neurons of the dorsomedial hypothalamus in chronic hyperphagic models

AU - Lee, Shin J.

AU - Kirigiti, Melissa

AU - Lindsley, Sarah R.

AU - Loche, Alberto

AU - Madden, Christopher (Chris)

AU - Morrison, Shaun

AU - Smith, M (Susan)

AU - Grove, Kevin

PY - 2013/6/1

Y1 - 2013/6/1

N2 - The dorsomedial hypothalamus (DMH) has long been implicated in feeding behavior and thermogenesis. The DMH contains orexigenic neuropeptide Y (NPY) neurons, but the role of these neurons in the control of energy homeostasis is not well understood. NPY expression in the DMH is low under normal conditions in adult rodents but is significantly increased during chronic hyperphagic conditions such as lactation and diet-induced obesity (DIO). To understand better the role of DMH-NPY neurons, we characterized the efferent projections of DMH-NPY neurons using the anterograde tracer biotinylated dextran amine (BDA) in lactating rats and DIO mice. In both models, BDA- and NPY-colabeled fibers were limited mainly to the hypothalamus, including the paraventricular nucleus of the hypothalamus (PVH), lateral hypothalamus/perifornical area (LH/PFA), and anteroventral periventricular nucleus (AVPV). Specifically in lactating rats, BDA-and NPY-colabeled axonal swellings were in close apposition to cocaine- and amphetamine-regulated transcript (CART)-expressing neurons in the PVH and AVPV. Although the DMH neurons project to the rostral raphe pallidus (rRPa), these projections did not contain NPY immunoreactivity in either the lactating rat or the DIO mouse. Instead, the majority of BDA-labeled fibers in the rRPa were orexin positive. Furthermore, DMH-NPY projections were not observed within the nucleus of the solitary tract (NTS), another brainstem site critical for the regulation of sympathetic outflow. The present data suggest that NPY expression in the DMH during chronic hyperphagic conditions plays important roles in feeding behavior and thermogenesis by modulating neuronal functions within the hypothalamus, but not in the brainstem. J. Comp. Neurol. 521:1891-1914, 2013.

AB - The dorsomedial hypothalamus (DMH) has long been implicated in feeding behavior and thermogenesis. The DMH contains orexigenic neuropeptide Y (NPY) neurons, but the role of these neurons in the control of energy homeostasis is not well understood. NPY expression in the DMH is low under normal conditions in adult rodents but is significantly increased during chronic hyperphagic conditions such as lactation and diet-induced obesity (DIO). To understand better the role of DMH-NPY neurons, we characterized the efferent projections of DMH-NPY neurons using the anterograde tracer biotinylated dextran amine (BDA) in lactating rats and DIO mice. In both models, BDA- and NPY-colabeled fibers were limited mainly to the hypothalamus, including the paraventricular nucleus of the hypothalamus (PVH), lateral hypothalamus/perifornical area (LH/PFA), and anteroventral periventricular nucleus (AVPV). Specifically in lactating rats, BDA-and NPY-colabeled axonal swellings were in close apposition to cocaine- and amphetamine-regulated transcript (CART)-expressing neurons in the PVH and AVPV. Although the DMH neurons project to the rostral raphe pallidus (rRPa), these projections did not contain NPY immunoreactivity in either the lactating rat or the DIO mouse. Instead, the majority of BDA-labeled fibers in the rRPa were orexin positive. Furthermore, DMH-NPY projections were not observed within the nucleus of the solitary tract (NTS), another brainstem site critical for the regulation of sympathetic outflow. The present data suggest that NPY expression in the DMH during chronic hyperphagic conditions plays important roles in feeding behavior and thermogenesis by modulating neuronal functions within the hypothalamus, but not in the brainstem. J. Comp. Neurol. 521:1891-1914, 2013.

KW - Food intake

KW - Hypothalamus

KW - Lactation

KW - Obesity

KW - Thermogenesis

UR - http://www.scopus.com/inward/record.url?scp=84875821516&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84875821516&partnerID=8YFLogxK

U2 - 10.1002/cne.23265

DO - 10.1002/cne.23265

M3 - Article

C2 - 23172177

AN - SCOPUS:84875821516

VL - 521

SP - 1891

EP - 1914

JO - Journal of Comparative Neurology

JF - Journal of Comparative Neurology

SN - 0021-9967

IS - 8

ER -