Effects of retinoic acid treatment on release of arachidonic acid by chondrogenic cells in response to ionophore A23187

Paul O. Moon, Kenneth P. Chepenik, Devendra M. Kochhar

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

Chondrogenic differentiation in mouse limb bud mesenchymal cells cultured at high density was suppressed by supplementation of the medium with retinoic acid in a dose-dependent fashion. Cells prelabeled with (3H) arachidonic acid were treated with 0.3 ug/ml retinoic acid. Treatment with retinoic acid increased the (3H) fatty acid in the triglyceride fraction. Furthermore, treatment with retinoic acid enhanced the release of (3H) fatty acid upon stimulation of these cells with the divalent ionophore A23187. These data permit the suggestion that there may be a correlation between altered lipid metabolism and retinoic acid's ability to disrupt chondrogenic differentiation.

Original languageEnglish (US)
Pages (from-to)1445-1450
Number of pages6
JournalLife Sciences
Volume38
Issue number16
DOIs
StatePublished - Apr 21 1986

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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