Effects of receptor occupancy on [3H]dexamethasone binding to circulating leukocytes

T. Murakami, D. D. Brandon, D. L. Loriaux, M. B. Lipsett

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

To determine the effect of steroid occupancy of the glucocorticoid receptor on the measurement of total receptor concentration using Scatchard plot of [3H]dexamethasone (Dex) binding to circulating leukocytes, leukocytes were incubated with 10-7 M cortisol or 10-9 M Dex prior to [3H]Dex binding study. Total binding capacity calculated from Scatchard plot analysis of [3H]Dex binding was not significantly reduced after pre-incubation with cortisol. However, total binding capacity was significantly lower after pre-incubation with Dex. These data suggest that total glucocorticoid receptor concentration can be measured from a 3 hr incubation of leukocytes obtained from untreated patients.

Original languageEnglish (US)
Pages (from-to)376-380
Number of pages5
JournalActa Endocrinologica
Volume94
Issue number3
StatePublished - Jan 1 1980

ASJC Scopus subject areas

  • Endocrinology

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