Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum

Michael Rutten, Hwa Tzong Chern, C. Diane Moore, James Hamilton, Laurence Y. Cheung

Research output: Contribution to journalArticle

Abstract

The effects of bovine parathyroid hormone (bPTH1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.

Original languageEnglish (US)
Pages (from-to)241-246
Number of pages6
JournalGeneral and Comparative Endocrinology
Volume75
Issue number2
DOIs
StatePublished - 1989
Externally publishedYes

Fingerprint

Necturus maculosus
Pyloric Antrum
parathyroid hormone
cyclic AMP
Parathyroid Hormone
Cyclic AMP
stomach
1-Methyl-3-isobutylxanthine
mucosa
Mucous Membrane
In Vitro Techniques
adenylate cyclase
Phosphodiesterase Inhibitors
Adenylyl Cyclases
pretreatment

ASJC Scopus subject areas

  • Endocrinology

Cite this

Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum. / Rutten, Michael; Chern, Hwa Tzong; Moore, C. Diane; Hamilton, James; Cheung, Laurence Y.

In: General and Comparative Endocrinology, Vol. 75, No. 2, 1989, p. 241-246.

Research output: Contribution to journalArticle

Rutten, Michael ; Chern, Hwa Tzong ; Moore, C. Diane ; Hamilton, James ; Cheung, Laurence Y. / Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum. In: General and Comparative Endocrinology. 1989 ; Vol. 75, No. 2. pp. 241-246.
@article{a6971d0264cb42948d4e54716e7eecae,
title = "Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum",
abstract = "The effects of bovine parathyroid hormone (bPTH1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.",
author = "Michael Rutten and Chern, {Hwa Tzong} and Moore, {C. Diane} and James Hamilton and Cheung, {Laurence Y.}",
year = "1989",
doi = "10.1016/0016-6480(89)90076-2",
language = "English (US)",
volume = "75",
pages = "241--246",
journal = "General and Comparative Endocrinology",
issn = "0016-6480",
publisher = "Academic Press Inc.",
number = "2",

}

TY - JOUR

T1 - Effects of parathyroid hormone on cyclic-AMP concentrations of in vitro Necturus maculosus gastric antrum

AU - Rutten, Michael

AU - Chern, Hwa Tzong

AU - Moore, C. Diane

AU - Hamilton, James

AU - Cheung, Laurence Y.

PY - 1989

Y1 - 1989

N2 - The effects of bovine parathyroid hormone (bPTH1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.

AB - The effects of bovine parathyroid hormone (bPTH1-84) on the stimulation of intracellular cyclic-AMP [cAMP] were investigated in an in vitro preparation of Necturus maculosus antral mucosa. When the antrum was exposed to 1, 5, 10, or 100 nM bPTH1-84, there was an ∼2-fold nonlinear increase in tissue [cAMP] over basal values. The pretreatment of the antral mucosa with 1 mM isobutylmethylxanthine (IBMX, a phosphodiesterase inhibitor) increased with detectability of mucosal [cAMP]. The addition of 1, 5, 10, or 100 nM bPTH1-84 to tissues pretreated with IBMX resulted in an ∼3.5-fold linear increase in mucosal [cAMP] over basal values. The time course of the generation of mucosal cAMP to 10 nM bPTH1-84 resulted in a small but significant transient increase at 2.5 min after the addition of bPTH1-84 but no change in the medium [cAMP]. In tissues pretreated with 1 mM IBMX the response to 10 nM bPTH1-84 was a large biphasic increase of [cAMP] at 2.5 min that progressively declined to near basal values by 15 min. There was also a significant sustained increase in the [cAMP] in the bathing medium at 2.5 min of tissues pretreated with IBMX followed by 10 nM bPTH1-84. These results suggest the presence ofan adenylate cyclase that can be activated by a mammalian bPTH1-84 in elevating intracellular cAMP levels in the N. maculosus antral mucosa.

UR - http://www.scopus.com/inward/record.url?scp=0024708579&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024708579&partnerID=8YFLogxK

U2 - 10.1016/0016-6480(89)90076-2

DO - 10.1016/0016-6480(89)90076-2

M3 - Article

VL - 75

SP - 241

EP - 246

JO - General and Comparative Endocrinology

JF - General and Comparative Endocrinology

SN - 0016-6480

IS - 2

ER -