Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes

Keith S. Whitacre, David Seifer, Chad I. Friedman, Serdar Coskun, Elizabeth A. Kennard, Moon H. Kim, Baha M. Alak

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60% versus 48%, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or

Original languageEnglish (US)
Pages (from-to)1015-1021
Number of pages7
JournalFertility and Sterility
Volume70
Issue number6
DOIs
StatePublished - Dec 1998
Externally publishedYes

Fingerprint

Menstrual Cycle
Oocytes
Gynecologic Surgical Procedures
Metaphase
In Vitro Oocyte Maturation Techniques
Oocyte Retrieval
Follicular Phase
Luteal Phase
Ovariectomy
In Vitro Techniques
Ovary
Outcome Assessment (Health Care)
Research

Keywords

  • Germinal vesicle breakdown (GVBD)
  • Human oocyte
  • Menstrual cycle phase
  • Metaphase II (MII)
  • Oocyte maturation

ASJC Scopus subject areas

  • Obstetrics and Gynecology

Cite this

Whitacre, K. S., Seifer, D., Friedman, C. I., Coskun, S., Kennard, E. A., Kim, M. H., & Alak, B. M. (1998). Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes. Fertility and Sterility, 70(6), 1015-1021. https://doi.org/10.1016/S0015-0282(98)00339-2

Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes. / Whitacre, Keith S.; Seifer, David; Friedman, Chad I.; Coskun, Serdar; Kennard, Elizabeth A.; Kim, Moon H.; Alak, Baha M.

In: Fertility and Sterility, Vol. 70, No. 6, 12.1998, p. 1015-1021.

Research output: Contribution to journalArticle

Whitacre, KS, Seifer, D, Friedman, CI, Coskun, S, Kennard, EA, Kim, MH & Alak, BM 1998, 'Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes', Fertility and Sterility, vol. 70, no. 6, pp. 1015-1021. https://doi.org/10.1016/S0015-0282(98)00339-2
Whitacre, Keith S. ; Seifer, David ; Friedman, Chad I. ; Coskun, Serdar ; Kennard, Elizabeth A. ; Kim, Moon H. ; Alak, Baha M. / Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes. In: Fertility and Sterility. 1998 ; Vol. 70, No. 6. pp. 1015-1021.
@article{0a300d4703cb48fab92e34c864ad5a0b,
title = "Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes",
abstract = "Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60{\%} versus 48{\%}, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or",
keywords = "Germinal vesicle breakdown (GVBD), Human oocyte, Menstrual cycle phase, Metaphase II (MII), Oocyte maturation",
author = "Whitacre, {Keith S.} and David Seifer and Friedman, {Chad I.} and Serdar Coskun and Kennard, {Elizabeth A.} and Kim, {Moon H.} and Alak, {Baha M.}",
year = "1998",
month = "12",
doi = "10.1016/S0015-0282(98)00339-2",
language = "English (US)",
volume = "70",
pages = "1015--1021",
journal = "Fertility and Sterility",
issn = "0015-0282",
publisher = "Elsevier Inc.",
number = "6",

}

TY - JOUR

T1 - Effects of ovarian source, patient age, and menstrual cycle phase on in vitro maturation of immature human oocytes

AU - Whitacre, Keith S.

AU - Seifer, David

AU - Friedman, Chad I.

AU - Coskun, Serdar

AU - Kennard, Elizabeth A.

AU - Kim, Moon H.

AU - Alak, Baha M.

PY - 1998/12

Y1 - 1998/12

N2 - Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60% versus 48%, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or

AB - Objective: To determine the efficiency of in vitro maturation, expressed by nuclear maturation, of oocytes aspirated during gynecologic surgeries or collected from excised ovaries. To assess the effect of patient age and cycle phase at collection on the oocyte's ability to mature in vitro. To examine the time course of oocyte maturation in vitro. Design: Nuclear maturation based on patient criteria compared. Setting: University-based IVF program and research center. Patient(s): Consented patients undergoing gynecologic surgeries or patients undergoing oophorectomy. Intervention(s): Oocytes were maintained in culture for 48 hours and evaluated for maturation. Main Outcome Measure(s): Nuclear maturation evaluated as germinal vesicle breakdown (GVBD) or progression to the metaphase II (MII) stage. Result(s): A significantly higher percentage of oocytes collected during the follicular phase of the menstrual cycle underwent GVBD than did oocytes collected during the luteal phase (60% versus 48%, respectively). The percentage of oocytes reaching the MII stage, from these two groups, was not different. No statistically significant differences in maturation were observed in oocytes from different ovarian sources or from patients >40 or

KW - Germinal vesicle breakdown (GVBD)

KW - Human oocyte

KW - Menstrual cycle phase

KW - Metaphase II (MII)

KW - Oocyte maturation

UR - http://www.scopus.com/inward/record.url?scp=0032401004&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032401004&partnerID=8YFLogxK

U2 - 10.1016/S0015-0282(98)00339-2

DO - 10.1016/S0015-0282(98)00339-2

M3 - Article

VL - 70

SP - 1015

EP - 1021

JO - Fertility and Sterility

JF - Fertility and Sterility

SN - 0015-0282

IS - 6

ER -