Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions

In vivo and in vitro studies

George E. Billman, Yoshinori Nishijima, Andriy E. Belevych, Dmitry Terentyev, Ying Xu, Kaylan M. Haizlip, Michelle M. Monasky, Nitisha Hiranandani, William Harris, Sandor Gyorke, Cynthia A. Carnes, Paul M L Janssen

Research output: Contribution to journalArticle

34 Citations (Scopus)

Abstract

Since omega-3 polyunsaturated fatty acids (n-3 PUFAs) can alter ventricular myocyte calcium handling, these fatty acids could adversely affect cardiac contractile function, particularly following myocardial infarction. Therefore, 4 wk after myocardial infarction, dogs were randomly assigned to either placebo (corn oil, 1 g/day, n = 16) or n-3 PUFAs supplement [docosahexaenoic acid (DHA) + eicosapentaenoic acid (EPA) ethyl esters; 1, 2, or 4 g/day; n = 7, 8, and 12, respectively] groups. In vivo, ventricular function was evaluated by echocardiography before and after 3 mo of treatment. At the end of the 3-mo period, hearts were removed and in vitro function was evaluated using right ventricular trabeculae and isolated left ventricular myocytes. The treatment elicited significant (P <0.0001) dose-dependent increases (16.4-fold increase with 4 g/day) in left ventricular tissue and red blood cell n-3 PUFA levels (EPA + DHA, placebo, 0.42 ± 0.04; 1 g/day, 3.02 ± 0.23; 2 g/day, 3.63 ± 0.17; and 4 g/day, 6.97 ± 0.33%). Regardless of the dose, n-3 PUFA treatment did not alter ventricular function in the intact animal (e.g., 4 g/day, fractional shortening: pre, 42.9 ± 1.6 vs. post, 40.1 ± 1.7%; placebo: pre, 39.2 ± 1.3 vs. post, 38.4 ± 1.6%). The developed force per cross-sectional area, changes in length- and frequency-dependent behavior in contractile force, and the inotropic response to β-adrenoceptor activation were also similar for trabeculae obtained from placebo- or n-3 PUFA-treated dogs. Finally, calcium currents and calcium transients were the same in myocytes from n-3 PUFA- and placebo-treated dogs. Thus dietary n-3 PUFAs did not adversely alter either in vitro or in vivo ventricular contractile function in dogs with healed infarctions.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume298
Issue number4
DOIs
StatePublished - Apr 2010
Externally publishedYes

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Ventricular Function
Omega-3 Fatty Acids
Myocardial Infarction
Dogs
Placebos
Muscle Cells
Docosahexaenoic Acids
Calcium
Eicosapentaenoic Acid
Corn Oil
In Vitro Techniques
Unsaturated Fatty Acids
Adrenergic Receptors
Infarction
Echocardiography
Fatty Acids
Therapeutics
Erythrocytes

Keywords

  • Contractility
  • Diet
  • Force-frequency relationship
  • Frank-Starling mechanism
  • Heart failure
  • Sudden cardiac death

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)
  • Cardiology and Cardiovascular Medicine

Cite this

Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions : In vivo and in vitro studies. / Billman, George E.; Nishijima, Yoshinori; Belevych, Andriy E.; Terentyev, Dmitry; Xu, Ying; Haizlip, Kaylan M.; Monasky, Michelle M.; Hiranandani, Nitisha; Harris, William; Gyorke, Sandor; Carnes, Cynthia A.; Janssen, Paul M L.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 298, No. 4, 04.2010.

Research output: Contribution to journalArticle

Billman, GE, Nishijima, Y, Belevych, AE, Terentyev, D, Xu, Y, Haizlip, KM, Monasky, MM, Hiranandani, N, Harris, W, Gyorke, S, Carnes, CA & Janssen, PML 2010, 'Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions: In vivo and in vitro studies', American Journal of Physiology - Heart and Circulatory Physiology, vol. 298, no. 4. https://doi.org/10.1152/ajpheart.01065.2009
Billman, George E. ; Nishijima, Yoshinori ; Belevych, Andriy E. ; Terentyev, Dmitry ; Xu, Ying ; Haizlip, Kaylan M. ; Monasky, Michelle M. ; Hiranandani, Nitisha ; Harris, William ; Gyorke, Sandor ; Carnes, Cynthia A. ; Janssen, Paul M L. / Effects of dietary omega-3 fatty acids on ventricular function in dogs with healed myocardial infarctions : In vivo and in vitro studies. In: American Journal of Physiology - Heart and Circulatory Physiology. 2010 ; Vol. 298, No. 4.
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