Effects of costimulator on immune responses in vitro

J. Shaw, V. Monticone, Gordon Mills, V. Paetkau

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

We recently described a factor, costimulator, that is required for the concavalin A-induced proliferation of CBA mouse thymocytes in vitro. Using the costimulator dependence of mouse thymocytes as an assay, we have now determined that spleen cells from congenitally athymic (nude) BALB/c mice do not produce costimulator in reponse to Con A, and spleen cells depleted of Thy 1-positive cells do not respond to it in the presence of Con A. Thus, costimulator both requires thymus derived (Thy 1+) lymphocytes for its production and has an effect on this type of cell. (However, the costimulator producing and responsive cells may be different). Purified costimulator preparations are a source of the required second component for the stimulation of adult, CBA/J thymic lymphocytes by PHA, normally a poor mitogen for these cells. They also enhance the level of DNA synthesis in a mixed leukocyte reaction, and the specific generation of cytotoxic lymphocytes to allogeneic tumor cells in vitro. Costimulator is not H-2 restricted in its effects, and it is produced in mixed leukocyte reactions. Finally, it has been possible to grow normal, primary thymic lymphocytes in culture for about 20 days by adding partially purified costimulator to the cultures.

Original languageEnglish (US)
Pages (from-to)1974-1980
Number of pages7
JournalJournal of Immunology
Volume120
Issue number6
StatePublished - Jan 1 1978
Externally publishedYes

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Lymphocytes
Mixed Lymphocyte Culture Test
Thymocytes
Spleen
Inbred CBA Mouse
In Vitro Techniques
Mitogens
Thymus Gland
DNA
Neoplasms

ASJC Scopus subject areas

  • Immunology

Cite this

Shaw, J., Monticone, V., Mills, G., & Paetkau, V. (1978). Effects of costimulator on immune responses in vitro. Journal of Immunology, 120(6), 1974-1980.

Effects of costimulator on immune responses in vitro. / Shaw, J.; Monticone, V.; Mills, Gordon; Paetkau, V.

In: Journal of Immunology, Vol. 120, No. 6, 01.01.1978, p. 1974-1980.

Research output: Contribution to journalArticle

Shaw, J, Monticone, V, Mills, G & Paetkau, V 1978, 'Effects of costimulator on immune responses in vitro', Journal of Immunology, vol. 120, no. 6, pp. 1974-1980.
Shaw J, Monticone V, Mills G, Paetkau V. Effects of costimulator on immune responses in vitro. Journal of Immunology. 1978 Jan 1;120(6):1974-1980.
Shaw, J. ; Monticone, V. ; Mills, Gordon ; Paetkau, V. / Effects of costimulator on immune responses in vitro. In: Journal of Immunology. 1978 ; Vol. 120, No. 6. pp. 1974-1980.
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