Effects of Anti-CD4 Antibody: Enhancement of Lymph Node PPD-Memory T Cell Response

William J. Morrison, Norman J. Kennedy, Halina Offner, Arthur Vandenbark

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In vitro incubations of CD4+ T cells with anti-CD4 monoclonal antibody (Mab) demonstrated saturation binding and inhibited both PPD-stimulated proliferation and delayed-type hypersensitivity (DTH) after passive transfer of these cells into naive rats. In comparison, in vivo administration of anti-CD4 Mab into rats, 2 weeks after CFA immunization (PPD printing), saturated CD4 molecules expressed on T lymphocytes and depleted significant percentages of CD4+ T cells from blood, spleen, and lymph nodes, yet failed to cause significant inhibition of PPD-stimulated DTH ear swelling. Only repeated in vivo administration of anti-CD4 Mabs during the CFA/PPD antigen priming caused significant decreases (36 ± 5%) in PPD-stimulated DTH ear swelling. T cells isolated from the blood and spleen showed insignificant relative decreases in PPD-stimulated proliferation after anti-CD4 treatments. In contrast, PPD-stimulated proliferation of T cells isolated from the draining lymph nodes after anti-CD4 treatments showed dramatic increases in PPD reactivity. PPD stimulation of T cells isolated from the draining lymph nodes of anti-CD4 Mab-treated rats produced relative phenotypic changes in CD4 coexpressions with CD45RC (inverse memory), CD49d (inflammatory adhesion integrin VLA-4), and OX40 (CD4+ blast), representative of memory CD4+ T cell blasts with reduced capacity for endothelial infiltration. These data demonstrate that depletion of CD4+ T cells by anti-CD4 Mab injections, administered either during or after antigen priming, selectively enhanced memory responses in T lymphocytes in the draining lymph nodes.

Original languageEnglish (US)
Pages (from-to)106-112
Number of pages7
JournalCellular Immunology
Volume163
Issue number1
DOIs
StatePublished - Jun 1995

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Tuberculin
Anti-Idiotypic Antibodies
Lymph Nodes
T-Lymphocytes
Delayed Hypersensitivity
Monoclonal Antibodies
Ear
Spleen
Integrin alpha4beta1
Antigens
CD4 Antigens
Printing
Integrins
Immunization
Injections
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Cell Biology

Cite this

Effects of Anti-CD4 Antibody : Enhancement of Lymph Node PPD-Memory T Cell Response. / Morrison, William J.; Kennedy, Norman J.; Offner, Halina; Vandenbark, Arthur.

In: Cellular Immunology, Vol. 163, No. 1, 06.1995, p. 106-112.

Research output: Contribution to journalArticle

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