Effectiveness of photosensitive dye during uptake and redistribution

Xiao Y. He, Steven L. Jacques, Gary Gofstein

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Scopus citations

Abstract

The location of photosensitive dye within a cell will affect the efficacy of photodynamic therapy (PDT). This report demonstrates that during the first 3 hours of dye (Photofrin porfimer) uptake from a liquid medium, the dye is diffusely distributed within the cell. After 24 hours of dye uptake, the dye is localized in specific sites within the cell. Fluorescence spectroscopy demonstrated that the 3-hour dye's emission peak near 635 nm was slightly blue shifted for the localized 24-hour dye. This paper demonstrates that the diffuse dye (at 3 hours) is more effective for PDT than the localized dye (at 24 hours). The amount of diffuse dye within the cell that is required to achieve an LD50 (50% lethal dose) is 1.5-fold to 4.5- fold less than the amount of localized dye required, using the same light exposure. The range of 1.5 - 4.5-fold refers to the results for four cell lines.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages205-215
Number of pages11
ISBN (Print)0819407917
StatePublished - Dec 1 1992
Externally publishedYes
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy - Los Angeles, CA, USA
Duration: Jan 20 1992Jan 21 1992

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume1645
ISSN (Print)0277-786X

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy
CityLos Angeles, CA, USA
Period1/20/921/21/92

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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  • Cite this

    He, X. Y., Jacques, S. L., & Gofstein, G. (1992). Effectiveness of photosensitive dye during uptake and redistribution. In Proceedings of SPIE - The International Society for Optical Engineering (pp. 205-215). (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 1645). Publ by Int Soc for Optical Engineering.