Effectiveness of photosensitive dye during uptake and redistribution

Xiao Y. He, Steven Jacques, Gary Gofstein

Research output: Chapter in Book/Report/Conference proceedingConference contribution

6 Citations (Scopus)

Abstract

The location of photosensitive dye within a cell will affect the efficacy of photodynamic therapy (PDT). This report demonstrates that during the first 3 hours of dye (Photofrin porfimer) uptake from a liquid medium, the dye is diffusely distributed within the cell. After 24 hours of dye uptake, the dye is localized in specific sites within the cell. Fluorescence spectroscopy demonstrated that the 3-hour dye's emission peak near 635 nm was slightly blue shifted for the localized 24-hour dye. This paper demonstrates that the diffuse dye (at 3 hours) is more effective for PDT than the localized dye (at 24 hours). The amount of diffuse dye within the cell that is required to achieve an LD50 (50% lethal dose) is 1.5-fold to 4.5- fold less than the amount of localized dye required, using the same light exposure. The range of 1.5 - 4.5-fold refers to the results for four cell lines.

Original languageEnglish (US)
Title of host publicationProceedings of SPIE - The International Society for Optical Engineering
PublisherPubl by Int Soc for Optical Engineering
Pages205-215
Number of pages11
Volume1645
ISBN (Print)0819407917
StatePublished - 1992
Externally publishedYes
EventOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy - Los Angeles, CA, USA
Duration: Jan 20 1992Jan 21 1992

Other

OtherOptical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy
CityLos Angeles, CA, USA
Period1/20/921/21/92

Fingerprint

Dyes
dyes
Photodynamic therapy
cells
therapy
Fluorescence spectroscopy
cultured cells
Cells
dosage
fluorescence
Liquids
liquids
spectroscopy

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

He, X. Y., Jacques, S., & Gofstein, G. (1992). Effectiveness of photosensitive dye during uptake and redistribution. In Proceedings of SPIE - The International Society for Optical Engineering (Vol. 1645, pp. 205-215). Publ by Int Soc for Optical Engineering.

Effectiveness of photosensitive dye during uptake and redistribution. / He, Xiao Y.; Jacques, Steven; Gofstein, Gary.

Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1645 Publ by Int Soc for Optical Engineering, 1992. p. 205-215.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

He, XY, Jacques, S & Gofstein, G 1992, Effectiveness of photosensitive dye during uptake and redistribution. in Proceedings of SPIE - The International Society for Optical Engineering. vol. 1645, Publ by Int Soc for Optical Engineering, pp. 205-215, Optical Methods for Tumor Treatment and Detection: Mechanisms and Techniques in Photodynamic Therapy, Los Angeles, CA, USA, 1/20/92.
He XY, Jacques S, Gofstein G. Effectiveness of photosensitive dye during uptake and redistribution. In Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1645. Publ by Int Soc for Optical Engineering. 1992. p. 205-215
He, Xiao Y. ; Jacques, Steven ; Gofstein, Gary. / Effectiveness of photosensitive dye during uptake and redistribution. Proceedings of SPIE - The International Society for Optical Engineering. Vol. 1645 Publ by Int Soc for Optical Engineering, 1992. pp. 205-215
@inproceedings{c42562376d074e418e8fc5c69a9aaa20,
title = "Effectiveness of photosensitive dye during uptake and redistribution",
abstract = "The location of photosensitive dye within a cell will affect the efficacy of photodynamic therapy (PDT). This report demonstrates that during the first 3 hours of dye (Photofrin porfimer) uptake from a liquid medium, the dye is diffusely distributed within the cell. After 24 hours of dye uptake, the dye is localized in specific sites within the cell. Fluorescence spectroscopy demonstrated that the 3-hour dye's emission peak near 635 nm was slightly blue shifted for the localized 24-hour dye. This paper demonstrates that the diffuse dye (at 3 hours) is more effective for PDT than the localized dye (at 24 hours). The amount of diffuse dye within the cell that is required to achieve an LD50 (50{\%} lethal dose) is 1.5-fold to 4.5- fold less than the amount of localized dye required, using the same light exposure. The range of 1.5 - 4.5-fold refers to the results for four cell lines.",
author = "He, {Xiao Y.} and Steven Jacques and Gary Gofstein",
year = "1992",
language = "English (US)",
isbn = "0819407917",
volume = "1645",
pages = "205--215",
booktitle = "Proceedings of SPIE - The International Society for Optical Engineering",
publisher = "Publ by Int Soc for Optical Engineering",

}

TY - GEN

T1 - Effectiveness of photosensitive dye during uptake and redistribution

AU - He, Xiao Y.

AU - Jacques, Steven

AU - Gofstein, Gary

PY - 1992

Y1 - 1992

N2 - The location of photosensitive dye within a cell will affect the efficacy of photodynamic therapy (PDT). This report demonstrates that during the first 3 hours of dye (Photofrin porfimer) uptake from a liquid medium, the dye is diffusely distributed within the cell. After 24 hours of dye uptake, the dye is localized in specific sites within the cell. Fluorescence spectroscopy demonstrated that the 3-hour dye's emission peak near 635 nm was slightly blue shifted for the localized 24-hour dye. This paper demonstrates that the diffuse dye (at 3 hours) is more effective for PDT than the localized dye (at 24 hours). The amount of diffuse dye within the cell that is required to achieve an LD50 (50% lethal dose) is 1.5-fold to 4.5- fold less than the amount of localized dye required, using the same light exposure. The range of 1.5 - 4.5-fold refers to the results for four cell lines.

AB - The location of photosensitive dye within a cell will affect the efficacy of photodynamic therapy (PDT). This report demonstrates that during the first 3 hours of dye (Photofrin porfimer) uptake from a liquid medium, the dye is diffusely distributed within the cell. After 24 hours of dye uptake, the dye is localized in specific sites within the cell. Fluorescence spectroscopy demonstrated that the 3-hour dye's emission peak near 635 nm was slightly blue shifted for the localized 24-hour dye. This paper demonstrates that the diffuse dye (at 3 hours) is more effective for PDT than the localized dye (at 24 hours). The amount of diffuse dye within the cell that is required to achieve an LD50 (50% lethal dose) is 1.5-fold to 4.5- fold less than the amount of localized dye required, using the same light exposure. The range of 1.5 - 4.5-fold refers to the results for four cell lines.

UR - http://www.scopus.com/inward/record.url?scp=0027062256&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027062256&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:0027062256

SN - 0819407917

VL - 1645

SP - 205

EP - 215

BT - Proceedings of SPIE - The International Society for Optical Engineering

PB - Publ by Int Soc for Optical Engineering

ER -