Effect of nutritional stress on the hypothalamo-pituitary-gonadal axis in the growing male rat

Cecilia V. Compagnucci, Gabriela E. Compagnucci, Alejandro Lomniczi, Claudia Mohn, Irene Vacas, Elisa Cebral, Juan C. Elverdin, Silvia M. Friedman, Valeria Rettori, Patricia Mónica Boyer

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Background/Objective: Nutritional dwarfing (ND) consists of a decrease in weight and height gain and delayed onset of puberty. The aim of the present investigation was to study the modifications induced in male rats by the nutritional stress of a mere 20% reduction in food intake which, however, started immediately after weaning. Materials and Methods: At weaning, male Wistar rats were divided into two groups: Control (C) and ND. C rats were fed ad libitum with a balanced rodent diet. ND received 80% of the diet consumed by C for 4 weeks (T4); then they were fed ad libitum for another 4 (T8) and 8 weeks (T12). The rats were studied at T0, T4, T8 and T12 for the effects of nutritional stress and refeeding on nutritional status, body composition, hypothalamic-pituitary-gonadal axis, and sperm morphology and concentration. Results: ND body weight and length diminished vs. C (p <0.001). ND body fat percentage decreased 40% (p <0.001) without change in the percentage of body protein content. The hypothalamic content of LHRH did not change. However, FSH, LH and testosterone serum levels had significantly decreased (p <0.001) at T4 in ND rats. A 48.4% decrease in serum leptin in the ND group was observed at T4 (p <0.05). The absolute testicular and seminal vesicle weight was significantly decreased by ND at T4 (p <0.001). At T4 the percentage of anomalies of caudal spermatozoa increased in about 64% (p <0.001) of ND vs. C rats, despite the unchanged sperm concentrations. All parameters normalized during refeeding. Conclusion: In this model, a decrease in leptin due to nutritional stress could be responsible, at least in part, for the inhibition of reproductive function. Refeeding normalized all parameters studied. Copyright

Original languageEnglish (US)
Pages (from-to)153-162
Number of pages10
JournalNeuroImmunoModulation
Volume10
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

Spermatozoa
Leptin
Weaning
Delayed Puberty
Diet
Seminal Vesicles
Body Composition
Nutritional Status
Serum
Gonadotropin-Releasing Hormone
Weight Gain
Testosterone
Adipose Tissue
Wistar Rats
Rodentia
Eating
Body Weight
Weights and Measures
Control Groups
Proteins

Keywords

  • Food restriction
  • Leptin
  • LH
  • LHRH
  • Sperm morphology
  • Testosterone
  • Weanling rat

ASJC Scopus subject areas

  • Endocrinology
  • Neuroscience(all)
  • Immunology

Cite this

Compagnucci, C. V., Compagnucci, G. E., Lomniczi, A., Mohn, C., Vacas, I., Cebral, E., ... Boyer, P. M. (2002). Effect of nutritional stress on the hypothalamo-pituitary-gonadal axis in the growing male rat. NeuroImmunoModulation, 10(3), 153-162. https://doi.org/10.1159/000067177

Effect of nutritional stress on the hypothalamo-pituitary-gonadal axis in the growing male rat. / Compagnucci, Cecilia V.; Compagnucci, Gabriela E.; Lomniczi, Alejandro; Mohn, Claudia; Vacas, Irene; Cebral, Elisa; Elverdin, Juan C.; Friedman, Silvia M.; Rettori, Valeria; Boyer, Patricia Mónica.

In: NeuroImmunoModulation, Vol. 10, No. 3, 2002, p. 153-162.

Research output: Contribution to journalArticle

Compagnucci, CV, Compagnucci, GE, Lomniczi, A, Mohn, C, Vacas, I, Cebral, E, Elverdin, JC, Friedman, SM, Rettori, V & Boyer, PM 2002, 'Effect of nutritional stress on the hypothalamo-pituitary-gonadal axis in the growing male rat', NeuroImmunoModulation, vol. 10, no. 3, pp. 153-162. https://doi.org/10.1159/000067177
Compagnucci, Cecilia V. ; Compagnucci, Gabriela E. ; Lomniczi, Alejandro ; Mohn, Claudia ; Vacas, Irene ; Cebral, Elisa ; Elverdin, Juan C. ; Friedman, Silvia M. ; Rettori, Valeria ; Boyer, Patricia Mónica. / Effect of nutritional stress on the hypothalamo-pituitary-gonadal axis in the growing male rat. In: NeuroImmunoModulation. 2002 ; Vol. 10, No. 3. pp. 153-162.
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abstract = "Background/Objective: Nutritional dwarfing (ND) consists of a decrease in weight and height gain and delayed onset of puberty. The aim of the present investigation was to study the modifications induced in male rats by the nutritional stress of a mere 20{\%} reduction in food intake which, however, started immediately after weaning. Materials and Methods: At weaning, male Wistar rats were divided into two groups: Control (C) and ND. C rats were fed ad libitum with a balanced rodent diet. ND received 80{\%} of the diet consumed by C for 4 weeks (T4); then they were fed ad libitum for another 4 (T8) and 8 weeks (T12). The rats were studied at T0, T4, T8 and T12 for the effects of nutritional stress and refeeding on nutritional status, body composition, hypothalamic-pituitary-gonadal axis, and sperm morphology and concentration. Results: ND body weight and length diminished vs. C (p <0.001). ND body fat percentage decreased 40{\%} (p <0.001) without change in the percentage of body protein content. The hypothalamic content of LHRH did not change. However, FSH, LH and testosterone serum levels had significantly decreased (p <0.001) at T4 in ND rats. A 48.4{\%} decrease in serum leptin in the ND group was observed at T4 (p <0.05). The absolute testicular and seminal vesicle weight was significantly decreased by ND at T4 (p <0.001). At T4 the percentage of anomalies of caudal spermatozoa increased in about 64{\%} (p <0.001) of ND vs. C rats, despite the unchanged sperm concentrations. All parameters normalized during refeeding. Conclusion: In this model, a decrease in leptin due to nutritional stress could be responsible, at least in part, for the inhibition of reproductive function. Refeeding normalized all parameters studied. Copyright",
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AU - Vacas, Irene

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AU - Elverdin, Juan C.

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