Effect of EDTA on the solid phase clq immune complex assay

Michael P. Davey, Leonhard Korngold

Research output: Contribution to journalArticle

2 Scopus citations

Abstract

A solid-phase Clq immune complex (IC) assay (C1q-SP) is described. Wells of microtiter plates are coated with Clq, sera diluted 1:40 are added, and complexed IgG bound to the solid phase is detected by peroxidase-labeled protein A. This assay selectively binds aggregated human gammaglobulin and soluble IC prepared in vitro. Treatment of sera with EDTA prior to Clq-SP analysis interferes with the test.

Original languageEnglish (US)
Pages (from-to)275-277
Number of pages3
JournalInternational Archives of Allergy and Immunology
Volume67
Issue number3
DOIs
StatePublished - Jan 1 1982

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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