Early lens development in the zebrafish: A three-dimensional time-lapse analysis

Teri Greiling, John I. Clark

Research output: Contribution to journalArticle

51 Citations (Scopus)

Abstract

In vivo, high-resolution, time-lapse imaging characterized lens development in the zebrafish from 16 to 96 hr postfertilization (hpf). In zebrafish, the lens placode appeared in the head ectoderm, similar to mammals. Delamination of the surface ectoderm resulted in the formation of the lens mass, which progressed to a solid sphere of cells separating from the developing cornea at approximately 24 hpf. A lens vesicle was not observed and apoptosis was not a major factor in separation of the lens from the future cornea. Differentiation of primary fibers began in the lens mass followed by formation of the anterior epithelium after delamination was complete. Secondary fibers differentiated from elongating epithelial cells near the posterior pole. Quantification characterized three stages of lens growth. The study confirmed the advantages of live-cell imaging for three-dimensional quantitative structural characterization of the mechanism(s) responsible for cell differentiation in formation of a transparent, symmetric, and refractile lens.

Original languageEnglish (US)
Pages (from-to)2254-2265
Number of pages12
JournalDevelopmental Dynamics
Volume238
Issue number9
DOIs
StatePublished - Sep 21 2009
Externally publishedYes

Fingerprint

Zebrafish
Lenses
Ectoderm
Cornea
Time-Lapse Imaging
Three-Dimensional Imaging
Cell Differentiation
Mammals
Epithelium
Epithelial Cells
Head
Apoptosis
Growth

Keywords

  • 3D
  • Delamination
  • Development
  • Imaging
  • Lens
  • Lens mass
  • Zebrafish

ASJC Scopus subject areas

  • Developmental Biology

Cite this

Early lens development in the zebrafish : A three-dimensional time-lapse analysis. / Greiling, Teri; Clark, John I.

In: Developmental Dynamics, Vol. 238, No. 9, 21.09.2009, p. 2254-2265.

Research output: Contribution to journalArticle

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