Doxycycline treatment reduces ischemic brain damage in transient middle cerebral artery occlusion in the rat

Wayne Clark, Nikola Lessov, Jeff D. Lauten, Kristin Hazel

Research output: Contribution to journalArticle

36 Citations (Scopus)

Abstract

Agents that inhibit leukocyte adhesion including intercellular adhesion molecule-1 antibodies (anti-ICAM-1) have shown beneficial effects in experimental central nervous system (CNS) ischemia. Doxycycline inhibits leukocyte function in vitro by binding divalent cations and reduces spinal cord reperfusion injury. The authors used a clinically relevant model of focal CNS reperfusion injury to test whether treatment with doxycycline would reduce cerebral ischemic damage and improve functional outcome. Reversible middle cerebral artery occlusion was produced in adult Sprague-Dawley rats by advancing a filament into the internal carotid artery for 2 h. Animals received either IP doxycycline (10 mg/kg) (N=13) or saline (N=11) 30 min before ischemia, followed by 10mg/kg every 8 h×6. Both functional assessment (5 point neurologic scale) and infarct volume was evaluated at 48 h. Functional efficacy: doxycycline 0.5±0.2 (mean±SE) vs control 1.3±0.3 (p=0.03). Infarct volume: doxycycline 56±18 mm3 vs control 158±44 mm3 (p=0.03); This protective effect supports the role of doxycycline in reducing CNS reperfusion injury.

Original languageEnglish (US)
Pages (from-to)103-108
Number of pages6
JournalJournal of Molecular Neuroscience
Volume9
Issue number2
DOIs
StatePublished - Oct 1997

Fingerprint

Doxycycline
Middle Cerebral Artery Infarction
Rats
Brain
Neurology
Reperfusion Injury
Nervous System Trauma
Central Nervous System
Intercellular Adhesion Molecule-1
Leukocytes
Ischemia
Functional assessment
Divalent Cations
Internal Carotid Artery
Spinal Cord Injuries
Nervous System
Sprague Dawley Rats
Anti-Idiotypic Antibodies
Animals
Adhesion

Keywords

  • Cerebral ischemia
  • doxycycline
  • leukocyte adhesion
  • therapy

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Biochemistry
  • Genetics
  • Neuroscience(all)

Cite this

Doxycycline treatment reduces ischemic brain damage in transient middle cerebral artery occlusion in the rat. / Clark, Wayne; Lessov, Nikola; Lauten, Jeff D.; Hazel, Kristin.

In: Journal of Molecular Neuroscience, Vol. 9, No. 2, 10.1997, p. 103-108.

Research output: Contribution to journalArticle

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