Down-regulation of the trypanosomatid signal recognition particle affects the biogenesis of polytopic membrane proteins but not of signal peptide-containing proteins

Yaniv Lustig, Yaron Vagima, Hanoch Goldshmidt, Avigail Erlanger, Vered Ozeri, James Vince, Malcolm J. McConville, Dennis M. Dwyer, Scott Landfear, Shulamit Michaeli

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

Protein translocation across the endoplasmic reticulum is mediated by the signal recognition particle (SRP). In this study, the SRP pathway in trypanosomatids was down-regulated by two approaches: RNA interference (RNAi) silencing of genes encoding SRP proteins in Trypanosoma brucei and overexpression of dominant-negative mutants of 7SL RNA in Leptomonas collosoma. The biogenesis of both signal peptide-containing proteins and polytopic membrane proteins was examined using endogenous and green fluorescent protein-fused proteins. RNAi silencing of SRP54 or SRP68 in T. brucei resulted in reduced levels of polytopic membrane proteins, but no effect on the level of signal peptide-containing proteins was observed. When SRP deficiency was achieved in L. collosoma by overexpression of dominant-negative mutated 7SL RNA, a major effect was observed on polytopic membrane proteins but not on signal peptide-containing proteins. This study included two trypanosomatid species, tested various protein substrates, and induced depletion of the SRP pathway by affecting either the levels of SRP binding proteins or that of SRP RNA. Our results demonstrate that, as in bacteria but in contrast to mammalian cells, the trypanosome SRP is mostly essential for the biogenesis of membrane proteins.

Original languageEnglish (US)
Pages (from-to)1865-1875
Number of pages11
JournalEukaryotic Cell
Volume6
Issue number10
DOIs
StatePublished - Oct 2007

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Signal Recognition Particle
Trypanosomatidae
Protein Sorting Signals
signal peptide
membrane proteins
Membrane Proteins
Down-Regulation
RNA Interference
Proteins
proteins
Trypanosoma brucei
Trypanosoma brucei brucei
RNA
RNA interference
Leptomonas
Trypanosomatina
Trypanosomiasis
protein transport
biogenesis
gene silencing

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Microbiology

Cite this

Down-regulation of the trypanosomatid signal recognition particle affects the biogenesis of polytopic membrane proteins but not of signal peptide-containing proteins. / Lustig, Yaniv; Vagima, Yaron; Goldshmidt, Hanoch; Erlanger, Avigail; Ozeri, Vered; Vince, James; McConville, Malcolm J.; Dwyer, Dennis M.; Landfear, Scott; Michaeli, Shulamit.

In: Eukaryotic Cell, Vol. 6, No. 10, 10.2007, p. 1865-1875.

Research output: Contribution to journalArticle

Lustig, Y, Vagima, Y, Goldshmidt, H, Erlanger, A, Ozeri, V, Vince, J, McConville, MJ, Dwyer, DM, Landfear, S & Michaeli, S 2007, 'Down-regulation of the trypanosomatid signal recognition particle affects the biogenesis of polytopic membrane proteins but not of signal peptide-containing proteins', Eukaryotic Cell, vol. 6, no. 10, pp. 1865-1875. https://doi.org/10.1128/EC.00134-07
Lustig, Yaniv ; Vagima, Yaron ; Goldshmidt, Hanoch ; Erlanger, Avigail ; Ozeri, Vered ; Vince, James ; McConville, Malcolm J. ; Dwyer, Dennis M. ; Landfear, Scott ; Michaeli, Shulamit. / Down-regulation of the trypanosomatid signal recognition particle affects the biogenesis of polytopic membrane proteins but not of signal peptide-containing proteins. In: Eukaryotic Cell. 2007 ; Vol. 6, No. 10. pp. 1865-1875.
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