Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds

Jia Yali, Pierre O. Bagnaninchi, Ying Yang, Alicia El Haj, Monica Hinds, Sean J. Kirkpatrick, Ruikang K. Wang

Research output: Contribution to journalArticle

27 Citations (Scopus)

Abstract

Establishing a relationship between perfusion rate and fluid shear stress in a 3D cell culture environment is an ongoing and challenging task faced by tissue engineers. We explore Doppler optical coherence tomography (DOCT) as a potential imaging tool for in situ monitoring of local fluid flow profiles inside porous chitosan scaffolds. From the measured fluid flow profiles, the fluid shear stresses are evaluated. We examine the localized fluid flow and shear stress within low- and high-porosity chitosan scaffolds, which are subjected to a constant input flow rate of 0.5mlmin -1. The DOCT results show that the behavior of the fluid flow and shear stress in micropores is strongly dependent on the micropore interconnectivity, porosity, and size of pores within the scaffold. For low-porosity and high-porosity chitosan scaffolds examined, the measured local fluid flow and shear stress varied from micropore to micropore, with a mean shear stress of 0.49±0.3dyncm -2 and 0.38±0.2dyncm -2, respectively. In addition, we show that the scaffold's porosity and interconnectivity can be quantified by combining analyses of the 3D structural and flow images obtained from DOCT.

Original languageEnglish (US)
Article number034014
JournalJournal of Biomedical Optics
Volume14
Issue number3
DOIs
StatePublished - 2009

Fingerprint

Optical tomography
Plastic flow
Scaffolds
shear stress
fluid flow
Shear stress
Flow of fluids
tomography
Porosity
porosity
Imaging techniques
Chitosan
Fluids
Cell culture
fluids
profiles
engineers
Flow rate
Tissue
flow velocity

Keywords

  • Doppler optical coherence tomography (DOCT)
  • interconnectivity
  • local fluid flow
  • porous scaffold
  • shear stress
  • tissue engineering

ASJC Scopus subject areas

  • Biomedical Engineering
  • Biomaterials
  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics

Cite this

Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds. / Yali, Jia; Bagnaninchi, Pierre O.; Yang, Ying; Haj, Alicia El; Hinds, Monica; Kirkpatrick, Sean J.; Wang, Ruikang K.

In: Journal of Biomedical Optics, Vol. 14, No. 3, 034014, 2009.

Research output: Contribution to journalArticle

Yali, Jia ; Bagnaninchi, Pierre O. ; Yang, Ying ; Haj, Alicia El ; Hinds, Monica ; Kirkpatrick, Sean J. ; Wang, Ruikang K. / Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds. In: Journal of Biomedical Optics. 2009 ; Vol. 14, No. 3.
@article{0c4e26183bcb4861a01595aeaeab2511,
title = "Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds",
abstract = "Establishing a relationship between perfusion rate and fluid shear stress in a 3D cell culture environment is an ongoing and challenging task faced by tissue engineers. We explore Doppler optical coherence tomography (DOCT) as a potential imaging tool for in situ monitoring of local fluid flow profiles inside porous chitosan scaffolds. From the measured fluid flow profiles, the fluid shear stresses are evaluated. We examine the localized fluid flow and shear stress within low- and high-porosity chitosan scaffolds, which are subjected to a constant input flow rate of 0.5mlmin -1. The DOCT results show that the behavior of the fluid flow and shear stress in micropores is strongly dependent on the micropore interconnectivity, porosity, and size of pores within the scaffold. For low-porosity and high-porosity chitosan scaffolds examined, the measured local fluid flow and shear stress varied from micropore to micropore, with a mean shear stress of 0.49±0.3dyncm -2 and 0.38±0.2dyncm -2, respectively. In addition, we show that the scaffold's porosity and interconnectivity can be quantified by combining analyses of the 3D structural and flow images obtained from DOCT.",
keywords = "Doppler optical coherence tomography (DOCT), interconnectivity, local fluid flow, porous scaffold, shear stress, tissue engineering",
author = "Jia Yali and Bagnaninchi, {Pierre O.} and Ying Yang and Haj, {Alicia El} and Monica Hinds and Kirkpatrick, {Sean J.} and Wang, {Ruikang K.}",
year = "2009",
doi = "10.1117/1.3130345",
language = "English (US)",
volume = "14",
journal = "Journal of Biomedical Optics",
issn = "1083-3668",
publisher = "SPIE",
number = "3",

}

TY - JOUR

T1 - Doppler optical coherence tomography imaging of local fluid flow and shear stress within microporous scaffolds

AU - Yali, Jia

AU - Bagnaninchi, Pierre O.

AU - Yang, Ying

AU - Haj, Alicia El

AU - Hinds, Monica

AU - Kirkpatrick, Sean J.

AU - Wang, Ruikang K.

PY - 2009

Y1 - 2009

N2 - Establishing a relationship between perfusion rate and fluid shear stress in a 3D cell culture environment is an ongoing and challenging task faced by tissue engineers. We explore Doppler optical coherence tomography (DOCT) as a potential imaging tool for in situ monitoring of local fluid flow profiles inside porous chitosan scaffolds. From the measured fluid flow profiles, the fluid shear stresses are evaluated. We examine the localized fluid flow and shear stress within low- and high-porosity chitosan scaffolds, which are subjected to a constant input flow rate of 0.5mlmin -1. The DOCT results show that the behavior of the fluid flow and shear stress in micropores is strongly dependent on the micropore interconnectivity, porosity, and size of pores within the scaffold. For low-porosity and high-porosity chitosan scaffolds examined, the measured local fluid flow and shear stress varied from micropore to micropore, with a mean shear stress of 0.49±0.3dyncm -2 and 0.38±0.2dyncm -2, respectively. In addition, we show that the scaffold's porosity and interconnectivity can be quantified by combining analyses of the 3D structural and flow images obtained from DOCT.

AB - Establishing a relationship between perfusion rate and fluid shear stress in a 3D cell culture environment is an ongoing and challenging task faced by tissue engineers. We explore Doppler optical coherence tomography (DOCT) as a potential imaging tool for in situ monitoring of local fluid flow profiles inside porous chitosan scaffolds. From the measured fluid flow profiles, the fluid shear stresses are evaluated. We examine the localized fluid flow and shear stress within low- and high-porosity chitosan scaffolds, which are subjected to a constant input flow rate of 0.5mlmin -1. The DOCT results show that the behavior of the fluid flow and shear stress in micropores is strongly dependent on the micropore interconnectivity, porosity, and size of pores within the scaffold. For low-porosity and high-porosity chitosan scaffolds examined, the measured local fluid flow and shear stress varied from micropore to micropore, with a mean shear stress of 0.49±0.3dyncm -2 and 0.38±0.2dyncm -2, respectively. In addition, we show that the scaffold's porosity and interconnectivity can be quantified by combining analyses of the 3D structural and flow images obtained from DOCT.

KW - Doppler optical coherence tomography (DOCT)

KW - interconnectivity

KW - local fluid flow

KW - porous scaffold

KW - shear stress

KW - tissue engineering

UR - http://www.scopus.com/inward/record.url?scp=70349243319&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=70349243319&partnerID=8YFLogxK

U2 - 10.1117/1.3130345

DO - 10.1117/1.3130345

M3 - Article

C2 - 19566307

AN - SCOPUS:70349243319

VL - 14

JO - Journal of Biomedical Optics

JF - Journal of Biomedical Optics

SN - 1083-3668

IS - 3

M1 - 034014

ER -