Dopaminergic signaling in the cochlea

Receptor expression patterns and deletion phenotypes

Stéphane F. Maison, Xiao Ping Liu, Ruth Anne Eatock, David R. Sibley, David Grandy, M. Charles Liberman

    Research output: Contribution to journalArticle

    30 Citations (Scopus)

    Abstract

    Pharmacological studies suggest that dopamine release from lateral olivocochlear efferent neurons suppresses spontaneous and soundevoked activity in cochlear nerve fibers and helps control noise-induced excitotoxicity; however, the literature on cochlear expression and localization of dopamine receptors is contradictory. To better characterize cochlear dopaminergic signaling, we studied receptor localization using immunohistochemistry or reverse transcriptase PCR and assessed histopathology, cochlear responses and olivocochlear function in mice with targeted deletion of each of the five receptor subtypes. In normal ears, D1, D2, and D5 receptors were detected in microdissected immature (postnatal days 10-13) spiral ganglion cells and outer hair cells but not inner hair cells. D4 was detected in spiral ganglion cells only. In whole cochlea samples from adults, transcripts for D1, D2, D4, and D5 were present, whereas D3 mRNA was never detected. D1 and D2 immunolabeling was localized to cochlear nerve fibers, near the first nodes of Ranvier (D2) and in the inner spiral bundle region (D1 and D2) where presynaptic olivocochlear terminals are found. No other receptor labeling was consistent. Cochlear function was normal in D3, D4, and D5 knock-outs. D1 and D2 knock-outs showed slight, but significant enhancement and suppression, respectively, of cochlear responses, both in the neural output [auditory brainstem response (ABR) wave 1] and in outer hair cell function [distortion product otoacoustic emissions (DPOAEs)]. Vulnerability to acoustic injury was significantly increased in D2, D4 and D5 lines: D1 could not be tested, and no differences were seen in D3 mutants, consistent with a lack of receptor expression. The increased vulnerability in D2 knock-outs was seen in DPOAEs, suggesting a role for dopamine in the outer hair cell area. In D4 and D5 knock-outs, the increased noise vulnerability was seen only in ABRs, consistent with a role for dopaminergic signaling in minimizing neural damage.

    Original languageEnglish (US)
    Pages (from-to)344-355
    Number of pages12
    JournalJournal of Neuroscience
    Volume32
    Issue number1
    DOIs
    StatePublished - Jan 4 2012

    Fingerprint

    Cochlea
    Outer Auditory Hair Cells
    Phenotype
    Spiral Ganglion
    Cochlear Nerve
    Nerve Fibers
    Inner Auditory Hair Cells
    Noise
    Dopamine
    Efferent Neurons
    Ranvier's Nodes
    Brain Stem Auditory Evoked Potentials
    Dopamine Receptors
    Presynaptic Terminals
    Reverse Transcriptase Polymerase Chain Reaction
    Acoustics
    Ear
    Immunohistochemistry
    Pharmacology
    Messenger RNA

    ASJC Scopus subject areas

    • Neuroscience(all)
    • Medicine(all)

    Cite this

    Maison, S. F., Liu, X. P., Eatock, R. A., Sibley, D. R., Grandy, D., & Charles Liberman, M. (2012). Dopaminergic signaling in the cochlea: Receptor expression patterns and deletion phenotypes. Journal of Neuroscience, 32(1), 344-355. https://doi.org/10.1523/JNEUROSCI.4720-11.2012

    Dopaminergic signaling in the cochlea : Receptor expression patterns and deletion phenotypes. / Maison, Stéphane F.; Liu, Xiao Ping; Eatock, Ruth Anne; Sibley, David R.; Grandy, David; Charles Liberman, M.

    In: Journal of Neuroscience, Vol. 32, No. 1, 04.01.2012, p. 344-355.

    Research output: Contribution to journalArticle

    Maison, SF, Liu, XP, Eatock, RA, Sibley, DR, Grandy, D & Charles Liberman, M 2012, 'Dopaminergic signaling in the cochlea: Receptor expression patterns and deletion phenotypes', Journal of Neuroscience, vol. 32, no. 1, pp. 344-355. https://doi.org/10.1523/JNEUROSCI.4720-11.2012
    Maison, Stéphane F. ; Liu, Xiao Ping ; Eatock, Ruth Anne ; Sibley, David R. ; Grandy, David ; Charles Liberman, M. / Dopaminergic signaling in the cochlea : Receptor expression patterns and deletion phenotypes. In: Journal of Neuroscience. 2012 ; Vol. 32, No. 1. pp. 344-355.
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