Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus

Ke Zhong Shen; Steven W. Johnson

doi:10.1097/00001756-200502080-00021

Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus

Ke Zhong Shen, Steven W. Johnson

Neurology

Research output: Contribution to journal › Article › peer-review

44 Scopus citations

Abstract

We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABA_A agonist isoguvacine (20 μM) and the GABA_B agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.

Original language	English (US)
Pages (from-to)	171-174
Number of pages	4
Journal	NeuroReport
Volume	16
Issue number	2
DOIs	https://doi.org/10.1097/00001756-200502080-00021
State	Published - Feb 8 2005

Keywords

6-OHDA
AMPA
Baclofen
Brain slice
Isoguvacine
NMDA

ASJC Scopus subject areas

Neuroscience(all)

Access to Document

10.1097/00001756-200502080-00021

Cite this

@article{55116b5f1925482894e426e20ff0dddf,

title = "Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus",

abstract = "We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.",

keywords = "6-OHDA, AMPA, Baclofen, Brain slice, Isoguvacine, NMDA",

author = "Shen, {Ke Zhong} and Johnson, {Steven W.}",

year = "2005",

month = feb,

day = "8",

doi = "10.1097/00001756-200502080-00021",

language = "English (US)",

volume = "16",

pages = "171--174",

journal = "NeuroReport",

issn = "0959-4965",

publisher = "Lippincott Williams and Wilkins",

number = "2",

}

TY - JOUR

T1 - Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus

AU - Shen, Ke Zhong

AU - Johnson, Steven W.

PY - 2005/2/8

Y1 - 2005/2/8

N2 - We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.

AB - We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.

KW - 6-OHDA

KW - AMPA

KW - Baclofen

KW - Brain slice

KW - Isoguvacine

KW - NMDA

UR - http://www.scopus.com/inward/record.url?scp=13844251858&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=13844251858&partnerID=8YFLogxK

U2 - 10.1097/00001756-200502080-00021

DO - 10.1097/00001756-200502080-00021

M3 - Article

C2 - 15671871

AN - SCOPUS:13844251858

VL - 16

SP - 171

EP - 174

JO - NeuroReport

JF - NeuroReport

SN - 0959-4965

IS - 2

ER -

Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus

Abstract

Keywords

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this