Abstract
We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 171-174 |
| Number of pages | 4 |
| Journal | NeuroReport |
| Volume | 16 |
| Issue number | 2 |
| DOIs | |
| State | Published - Feb 8 2005 |
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Keywords
- 6-OHDA
- AMPA
- Baclofen
- Brain slice
- Isoguvacine
- NMDA
ASJC Scopus subject areas
- Neuroscience(all)
Cite this
Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus. / Shen, Ke-Zhong; Johnson, Steven.
In: NeuroReport, Vol. 16, No. 2, 08.02.2005, p. 171-174.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Dopamine depletion alters responses to glutamate and GABA in the rat subthalamic nucleus
AU - Shen, Ke-Zhong
AU - Johnson, Steven
PY - 2005/2/8
Y1 - 2005/2/8
N2 - We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.
AB - We used whole-cell recordings to compare currents evoked by glutamate and GABA receptor agonists in subthalamic nucleus neurons located ipsilateral and contralateral to unilateral 6-hydroxydopamine (6-OHDA) injections into the substantia nigra zona compacta. The ratio of currents evoked by AMPA (0.6 μM) and NMDA (20 μM) was significantly greater in neurons recorded ipsilateral to 6-OHDA lesions compared with the ratio of currents recorded in control (contralateral) neurons. Both the GABAA agonist isoguvacine (20 μM) and the GABAB agonist baclofen (10 μM) evoked significantly greater outward currents in subthalamic nucleus neurons ipsilateral to the lesion compared to contralateral neurons. We conclude that chronic dopamine depletion upregulates expression of GABA receptors and shifts the functional expression of ionotropic glutamate receptor subtype from NMDA to AMPA receptors in subthalamic nucleus.
KW - 6-OHDA
KW - AMPA
KW - Baclofen
KW - Brain slice
KW - Isoguvacine
KW - NMDA
UR - http://www.scopus.com/inward/record.url?scp=13844251858&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=13844251858&partnerID=8YFLogxK
U2 - 10.1097/00001756-200502080-00021
DO - 10.1097/00001756-200502080-00021
M3 - Article
C2 - 15671871
AN - SCOPUS:13844251858
VL - 16
SP - 171
EP - 174
JO - NeuroReport
JF - NeuroReport
SN - 0959-4965
IS - 2
ER -