DNA vaccine strategies

Candidates for immune modulation and immunization regimens

Nicole A. Doria-Rose, Nancy Haigwood

    Research output: Contribution to journalArticle

    85 Citations (Scopus)

    Abstract

    DNA vaccine strategies can differ greatly, with significant effects on the outcome of immunization. In this article, we discuss plasmid design strategies and vaccine regimens. Effectiveness against a pathogen can be affected by the choice of antigen and inclusion of multiple antigens. Gene expression and the resulting immune response can be improved by gene modification and choice of promoters. In designing vaccine regimens, one must consider further dose, timing of doses, adjuvants, and routes of vaccination. Many vaccines are enhanced by combining DNA with other vaccines in "prime-boost" regimens, in which the second vaccine is often a recombinant viral vector or purified protein subunit. Prime-boost vaccines including DNA can elicit immune responses that differ in magnitude, quality, and balance of cellular and humoral responses from those elicited by single components and thus provide further enhancement for DNA immunizations.

    Original languageEnglish (US)
    Pages (from-to)207-216
    Number of pages10
    JournalMethods
    Volume31
    Issue number3
    DOIs
    StatePublished - Nov 1 2003

    Fingerprint

    Immunization
    DNA Vaccines
    Vaccines
    Modulation
    Antigens
    DNA
    Protein Subunits
    Pathogens
    Gene expression
    Vaccination
    Plasmids
    Genes
    Gene Expression

    Keywords

    • Adjuvant
    • Antigen
    • Plasmid
    • Prime-boost
    • Promoter

    ASJC Scopus subject areas

    • Molecular Biology

    Cite this

    DNA vaccine strategies : Candidates for immune modulation and immunization regimens. / Doria-Rose, Nicole A.; Haigwood, Nancy.

    In: Methods, Vol. 31, No. 3, 01.11.2003, p. 207-216.

    Research output: Contribution to journalArticle

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