DNA synthesis induced by some but not all growth factors requires Src family protein tyrosine kinases

S. Roche, M. Koegl, M. V. Barone, M. F. Roussel, Sara Courtneidge

Research output: Contribution to journalArticle

212 Citations (Scopus)

Abstract

The Src family of protein tyrosine kinases have been implicated in the response of cells to several ligands. These include platelet-derived growth factor (PDGF), epidermal growth factor (EGF), and colony stimulating factor type 1 (CSF-1, in macrophages and in fibroblasts engineered to express the receptor). We recently described a microinjection approach which we used to demonstrate that Src family kinases are required for PDGF-induced S phase entry of fibroblasts. We now use this approach to ask whether other ligands also require Src kinases to stimulate cells to replicate DNA. An antibody specific for the carboxy terminus of Src, Fyn, and Yes (anti-cst.1) inhibited Src kinase activity in vitro and caused morphological reversion of Src transformed cells in vivo. Microinjection of this antibody was used to demonstrate that Src kinases were required for both CSF-1 and EGF to drive cells into the S phase. Expression of a kinase-inactive form of Src family kinases also prevented EGF- and CSF-1-stimulated DNA synthesis. However, even though the Src family kinases were necessary for both PDGF- and EGF-induced DNA synthesis in Swiss 3T3 cells, the responses to two other potent growth factors for these cells, lysophosphatidic acid and bombesin, were unaffected by the neutralizing antibodies. Therefore, some but not all growth factors required functional Src family kinases to transmit mitogenic responses.

Original languageEnglish (US)
Pages (from-to)1102-1109
Number of pages8
JournalMolecular and Cellular Biology
Volume15
Issue number2
StatePublished - 1995
Externally publishedYes

Fingerprint

src-Family Kinases
Intercellular Signaling Peptides and Proteins
DNA
Macrophage Colony-Stimulating Factor
Epidermal Growth Factor
Platelet-Derived Growth Factor
Microinjections
S Phase
Fibroblasts
Swiss 3T3 Cells
Ligands
Bombesin
Antibodies
Neutralizing Antibodies
Phosphotransferases
Macrophages

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

DNA synthesis induced by some but not all growth factors requires Src family protein tyrosine kinases. / Roche, S.; Koegl, M.; Barone, M. V.; Roussel, M. F.; Courtneidge, Sara.

In: Molecular and Cellular Biology, Vol. 15, No. 2, 1995, p. 1102-1109.

Research output: Contribution to journalArticle

Roche, S. ; Koegl, M. ; Barone, M. V. ; Roussel, M. F. ; Courtneidge, Sara. / DNA synthesis induced by some but not all growth factors requires Src family protein tyrosine kinases. In: Molecular and Cellular Biology. 1995 ; Vol. 15, No. 2. pp. 1102-1109.
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