DNA sequence organization of the β-globin complex in the BALB/c mouse

C. L. Jahn, C. A. Hutchison, S. J. Phillips, S. Weaver, Nancy Haigwood, C. F. Voliva, M. H. Edgell

Research output: Chapter in Book/Report/Conference proceedingChapter

113 Citations (Scopus)

Abstract

Clones containing five non-adult β-globin genes were isolated from a library of BALB/c DNA. Together, the newly cloned regions comprise a contiguous block of 32 kb of the mouse genome. Restriction mapping of genomic DNA established the physical linkage of these non-adult genes to the two adult β-globin genes as well as of the two adult genes to each other. Thus, this entire BALB/c β-globin complex consists of seven linked genes, all with the same transcriptional orientation, arrayed over 70 kb of DNA.The order of these genes is: 5'-εy3-βh0-βh1-βh2-βh3-β(major)-β(minor)-3'. Portions of each of the five newly identified genes have been sequenced. Only one, εy3, encodes a previously described globin. βh0 and βh1, which are closely related, differ significantly from both adult and embryonic globins. The βh2 sequence is more closely related to the adult genes than to the other non-adult genes. However, it is the sequence least homologous to both of these classes. The 5' half of the βh3 gene (preceding codon 75) has an aberrant structure. The rest of this sequence is intact, resembling an adult β-globin gene except for an inserted base at codon 90, resulting in a frameshift. Consequently, the βh3 sequence cannot be translated to produce a normal β-globin.

Original languageEnglish (US)
Title of host publicationCell
Pages159-168
Number of pages10
Volume21
Edition1
DOIs
StatePublished - 1980
Externally publishedYes

Fingerprint

Globins
Genes
Codon
DNA
Restriction Mapping
Gene Order
Sequence Homology
Clone Cells
Genome

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Jahn, C. L., Hutchison, C. A., Phillips, S. J., Weaver, S., Haigwood, N., Voliva, C. F., & Edgell, M. H. (1980). DNA sequence organization of the β-globin complex in the BALB/c mouse. In Cell (1 ed., Vol. 21, pp. 159-168) https://doi.org/10.1016/0092-8674(80)90123-3

DNA sequence organization of the β-globin complex in the BALB/c mouse. / Jahn, C. L.; Hutchison, C. A.; Phillips, S. J.; Weaver, S.; Haigwood, Nancy; Voliva, C. F.; Edgell, M. H.

Cell. Vol. 21 1. ed. 1980. p. 159-168.

Research output: Chapter in Book/Report/Conference proceedingChapter

Jahn, CL, Hutchison, CA, Phillips, SJ, Weaver, S, Haigwood, N, Voliva, CF & Edgell, MH 1980, DNA sequence organization of the β-globin complex in the BALB/c mouse. in Cell. 1 edn, vol. 21, pp. 159-168. https://doi.org/10.1016/0092-8674(80)90123-3
Jahn CL, Hutchison CA, Phillips SJ, Weaver S, Haigwood N, Voliva CF et al. DNA sequence organization of the β-globin complex in the BALB/c mouse. In Cell. 1 ed. Vol. 21. 1980. p. 159-168 https://doi.org/10.1016/0092-8674(80)90123-3
Jahn, C. L. ; Hutchison, C. A. ; Phillips, S. J. ; Weaver, S. ; Haigwood, Nancy ; Voliva, C. F. ; Edgell, M. H. / DNA sequence organization of the β-globin complex in the BALB/c mouse. Cell. Vol. 21 1. ed. 1980. pp. 159-168
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