DNA methylation is associated with altered gene expression in AMD.

Allan Hunter, Paul A. Spechler, Alyssa Cwanger, Ying Song, Zhe Zhang, Gui Shuang Ying, Anna Hunter, Edwin Dezoeten, Joshua L. Dunaief

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Age-related macular degeneration (AMD) is the leading cause of blindness in the elderly. Evidence suggests oxidative stress plays a role in the disease. To assess the potential contribution of epigenetic regulation of antioxidant genes relevant to AMD pathogenesis, we evaluated DNA methylation, a tissue-specific genetic modulation that affects gene expression. Using the Infinium HumanMethylation27 Illumina platform, we performed DNA bisulfite sequencing to compare the methylation status in postmortem retina pigment epithelium (RPE)/choroid between patients with AMD and age-matched controls. Gene expression was assessed with the Affymetrix Exon Array. TaqMan gene expression assays were used for relative quantification (RT-PCR) confirmation of the expression array results: Glutathione S-transferase isoform mu1 (GSTM1) and mu5 (GSTM5) promoter methylation was confirmed by CpG island bisulfite pyrosequencing. To assess protein levels and localization, we used Western analysis, immunohistochemistry, and immunofluorescence with murine and human samples. The mRNA levels of GSTM1 and GSTM5 were significantly reduced in AMD versus age-matched controls in RPE/choroid and neurosensory retina (NSR), which corresponded to hypermethylation of the GSTM1 promoter. mRNA and protein levels were decreased (RPE to a greater extent than NSR) in AMD postmortem samples, irrespective of age. Immunohistochemistry and immunofluorescence confirm the presence of the enzymes in the NSR and RPE. Comparison of DNA methylation, together with mRNA levels, revealed significant differences between AMD versus normal retinas. The evidence presented suggests that GSTM1 and GSTM5 undergo epigenetic repression in AMD RPE/choroid, which may increase susceptibility to oxidative stress in AMD retinas.

Original languageEnglish (US)
Pages (from-to)2089-2105
Number of pages17
JournalInvestigative ophthalmology & visual science
Volume53
Issue number4
DOIs
StatePublished - Jan 1 2012
Externally publishedYes

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Macular Degeneration
DNA Methylation
Retina
Gene Expression
Glutathione Transferase
Epithelium
Choroid
Protein Isoforms
Messenger RNA
Methylation
Fluorescent Antibody Technique
Epigenetic Repression
Oxidative Stress
Immunohistochemistry
CpG Islands
Blindness
DNA Sequence Analysis
Epigenomics
Exons
Proteins

ASJC Scopus subject areas

  • Ophthalmology
  • Sensory Systems
  • Cellular and Molecular Neuroscience

Cite this

Hunter, A., Spechler, P. A., Cwanger, A., Song, Y., Zhang, Z., Ying, G. S., ... Dunaief, J. L. (2012). DNA methylation is associated with altered gene expression in AMD. Investigative ophthalmology & visual science, 53(4), 2089-2105. https://doi.org/10.1167/iovs.11-8449

DNA methylation is associated with altered gene expression in AMD. / Hunter, Allan; Spechler, Paul A.; Cwanger, Alyssa; Song, Ying; Zhang, Zhe; Ying, Gui Shuang; Hunter, Anna; Dezoeten, Edwin; Dunaief, Joshua L.

In: Investigative ophthalmology & visual science, Vol. 53, No. 4, 01.01.2012, p. 2089-2105.

Research output: Contribution to journalArticle

Hunter, A, Spechler, PA, Cwanger, A, Song, Y, Zhang, Z, Ying, GS, Hunter, A, Dezoeten, E & Dunaief, JL 2012, 'DNA methylation is associated with altered gene expression in AMD.', Investigative ophthalmology & visual science, vol. 53, no. 4, pp. 2089-2105. https://doi.org/10.1167/iovs.11-8449
Hunter, Allan ; Spechler, Paul A. ; Cwanger, Alyssa ; Song, Ying ; Zhang, Zhe ; Ying, Gui Shuang ; Hunter, Anna ; Dezoeten, Edwin ; Dunaief, Joshua L. / DNA methylation is associated with altered gene expression in AMD. In: Investigative ophthalmology & visual science. 2012 ; Vol. 53, No. 4. pp. 2089-2105.
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