Division of labor among the α6β4 integrin, β1 integrins, and an E3 laminin receptor to signal morphogenesis and β-casein expression in mammary epithelial cells

John Muschler, André Lochter, Calvin D. Roskelley, Peter Yurchenco, Mina J. Bissell

Research output: Contribution to journalArticle

97 Citations (Scopus)

Abstract

Contact of cultured mammary epithelial cells with the basement membrane protein laminin induces multiple responses, including cell shape changes, growth arrest, and, in the presence of prolactin, transcription of the milk protein β-casein. We sought to identify the specific laminin receptor(s) mediating the multiple cell responses to laminin. Using assays with clonal mammary epithelial cells, we reveal distinct functions for the α6β4 integrin, β1 integrins, and an E3 laminin receptor. Signals from laminin for β-casein expression were inhibited in the presence of function-blocking antibodies against both the α6 and β1 integrin subunits and by the laminin E3 fragment. The α6-blocking antibody perturbed signals mediated by the α6β4 integrin, and the β1-blocking antibody perturbed signals mediated by another integrin, the α subunit(s) of which remains to be determined. Neither α6- nor β1-blocking antibodies perturbed the cell shape changes resulting from cell exposure to laminin. However, the E3 laminin fragment and heparin both inhibited cell shape changes induced by laminin, thereby implicating an E3 laminin receptor in this function. These results elucidate the multiplicity of cell-extracellular matrix interactions required to integrate cell structure and signaling and ultimately permit normal cell function.

Original languageEnglish (US)
Pages (from-to)2817-2828
Number of pages12
JournalMolecular Biology of the Cell
Volume10
Issue number9
StatePublished - Sep 1999
Externally publishedYes

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Laminin Receptors
Laminin
Caseins
Morphogenesis
Integrins
Breast
Epithelial Cells
Blocking Antibodies
Cell Shape
Milk Proteins
Basement Membrane
Prolactin
Extracellular Matrix
Heparin
Membrane Proteins
Growth

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Division of labor among the α6β4 integrin, β1 integrins, and an E3 laminin receptor to signal morphogenesis and β-casein expression in mammary epithelial cells. / Muschler, John; Lochter, André; Roskelley, Calvin D.; Yurchenco, Peter; Bissell, Mina J.

In: Molecular Biology of the Cell, Vol. 10, No. 9, 09.1999, p. 2817-2828.

Research output: Contribution to journalArticle

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