Diversity of voltage-gated sodium channels in the ascidian larval nervous system

Hitoshi Nagahora, Toshiaki Okada, Naoya Yahagi, Jayhong Andrew Chong, Gail Mandel, Yasushi Okamura

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

To gain insight into the origin of the molecular diversity of voltage-gated sodium channels (NaVs), a putative sodium channel gene (TuNa2) was cloned from the protochordate ascidian. TuNa2 showed two unusual features in its primary structure; (1) lysine in the P-region of the third repeat, a critical site determining ion selectivity, was changed to glutamic acid, predicting that the ionic permeability would not be rigidly sodium-selective (2) the III-IV linker, determinant of fast inactivation, was only weakly conserved. In contrast with a pan-neuronally expressed NaV (TuNa1), expression of TuNa2 was confined to subsets of neurons including motor neurons, suggesting that TuNa2 plays specialized roles in electrical activities unique to these neurons. Basic FGF, a neural inducer in the ascidian embryo, induces TuNa2 RNA expression in the ectodermal cells at lower doses than that required for TuNa1 gene expression. Thus, two types of NaV may play distinct roles and their gene expressions are controlled by distinct mechanisms. (C) 2000 Academic Press.

Original languageEnglish (US)
Pages (from-to)558-564
Number of pages7
JournalBiochemical and Biophysical Research Communications
Volume275
Issue number2
DOIs
StatePublished - Aug 28 2000
Externally publishedYes

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Voltage-Gated Sodium Channels
Urochordata
Neurology
Nervous System
Neurons
Gene Expression
Gene expression
Sodium Channels
Motor Neurons
Lysine
Glutamic Acid
Permeability
Embryonic Structures
Sodium
RNA
Ions
Genes

Keywords

  • Ascidian
  • bFGF
  • Ion selectivity
  • Motor neuron
  • Sodium channel

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Diversity of voltage-gated sodium channels in the ascidian larval nervous system. / Nagahora, Hitoshi; Okada, Toshiaki; Yahagi, Naoya; Chong, Jayhong Andrew; Mandel, Gail; Okamura, Yasushi.

In: Biochemical and Biophysical Research Communications, Vol. 275, No. 2, 28.08.2000, p. 558-564.

Research output: Contribution to journalArticle

Nagahora, Hitoshi ; Okada, Toshiaki ; Yahagi, Naoya ; Chong, Jayhong Andrew ; Mandel, Gail ; Okamura, Yasushi. / Diversity of voltage-gated sodium channels in the ascidian larval nervous system. In: Biochemical and Biophysical Research Communications. 2000 ; Vol. 275, No. 2. pp. 558-564.
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