TY - JOUR
T1 - Diverse Mn(II)-oxidizing bacteria isolated from submarine basalts at Loihi seamount
AU - Templeton, Alexis S.
AU - Staudigel, Hubert
AU - Tebo, Bradley M.
N1 - Funding Information:
We thank Brad Bailey, Hope Johnson, Carolyn Sheehan, and Dan Rogers for assistance in sample collection and processing during Pisces dives and aboard the K ′O′K . We also appreciate the guidance of Terry Kerby and the operational support provided by the Hawai’i Undersea Research Lab (HURL) Pisces submersible team. HURL is a part of NURP and funded by NOAA. In addition, we thank the Advanced Photon Source, in particular GeoSoilEnviroCARS at Sector 13, for the beamtime necessary to collect the XAS data. The APS and GSECARS are funded by DOE. Financial support for this research was provided by a National Science Foundation Microbial Biology Postdoctoral Fellowship (AST), an NSF-Ocean Sciences SGER (NSF-OCE #0218945), an NSF Collaborative Research Activities in Environmental Molecular Sciences (CHE #0089208) grant, and a NSF Biogeosciences grant (#OCE0433692).
PY - 2005/4
Y1 - 2005/4
N2 - Metal-oxidizing bacteria may play a key role in the submarine weathering of volcanic rocks and the formation of ferromanganese crusts. Putative fossil microbes encrusted in Mn oxide phases are commonly observed on volcanic glasses recovered from the deep ocean; however, no known Mn(II)-oxidizing bacteria have been directly identified or cultured from natural weathered basalts. To isolate epilithic Mn(II) oxidizing bacteria, we collected young, oxidized pillow basalts from the cold, outer portions of Loihi Seamount, and from nearby exposures of pillow basalts at South Point and Kealakekua Bay, HI. SEM imaging, EDS spectra and X-ray absorption spectroscopy data show that microbial biolfilms and associated Mn oxides were abundant on the basalt surfaces. Using a series of seawater-based media that range from highly oligotrophic to organic-rich, we have obtained 26 mesophilic, heterotrophic Mn(II)-oxidizing isolates dominated by α and γ-Proteobacteria, such as Sulfitobacter, Methylarcula and Pseudoatteromonas spp. Additional isolates include Microbulbifer, Alteromonas, Marinobacter, and Halomonas spp. None of the isolate, nor their closet relatives, were previosly recognized as Mn(II) oxidizing bacteria. The physiological function of Mn(II) oxidation is clearly spread amongst many phylogenetically diverse organisms colonizing basalt surfaces. Our findings support a biological catalist of Mn(II) oxidation during basalt-wearing, and suggest heterotrophic Mn(II) oxidixing bacteria may be ubiquitously associated with submarine glasses within epilithic and endolithic biofilms.
AB - Metal-oxidizing bacteria may play a key role in the submarine weathering of volcanic rocks and the formation of ferromanganese crusts. Putative fossil microbes encrusted in Mn oxide phases are commonly observed on volcanic glasses recovered from the deep ocean; however, no known Mn(II)-oxidizing bacteria have been directly identified or cultured from natural weathered basalts. To isolate epilithic Mn(II) oxidizing bacteria, we collected young, oxidized pillow basalts from the cold, outer portions of Loihi Seamount, and from nearby exposures of pillow basalts at South Point and Kealakekua Bay, HI. SEM imaging, EDS spectra and X-ray absorption spectroscopy data show that microbial biolfilms and associated Mn oxides were abundant on the basalt surfaces. Using a series of seawater-based media that range from highly oligotrophic to organic-rich, we have obtained 26 mesophilic, heterotrophic Mn(II)-oxidizing isolates dominated by α and γ-Proteobacteria, such as Sulfitobacter, Methylarcula and Pseudoatteromonas spp. Additional isolates include Microbulbifer, Alteromonas, Marinobacter, and Halomonas spp. None of the isolate, nor their closet relatives, were previosly recognized as Mn(II) oxidizing bacteria. The physiological function of Mn(II) oxidation is clearly spread amongst many phylogenetically diverse organisms colonizing basalt surfaces. Our findings support a biological catalist of Mn(II) oxidation during basalt-wearing, and suggest heterotrophic Mn(II) oxidixing bacteria may be ubiquitously associated with submarine glasses within epilithic and endolithic biofilms.
KW - Basalt
KW - Biofilm
KW - Manganese oxidation
UR - http://www.scopus.com/inward/record.url?scp=20644462572&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=20644462572&partnerID=8YFLogxK
U2 - 10.1080/01490450590945951
DO - 10.1080/01490450590945951
M3 - Article
AN - SCOPUS:20644462572
SN - 0149-0451
VL - 22
SP - 127
EP - 139
JO - Geomicrobiology Journal
JF - Geomicrobiology Journal
IS - 3-4
ER -