Distribution of the scaffolding proteins PSD-95, PSD-93, and SAP97 in isolated PSDs

Joseph A. DeGiorgis, James Galbraith, Ayse Dosemeci, Xiaobing Chen, Thomas S. Reese

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

We compared the distribution of three scaffolding proteins, all belonging to a family of membrane-associated guanylate kinases, thought to have key roles in the organization of the postsynaptic density (PSD). Isolated PSDs readily adhered to treated glass coverslips where they were labeled with immunogold and rotary shadowed for analysis by EM. The distribution of proteins within individual PSDs were measured by counting and mapping individual immunogold particles. PSD-95, as previously described, is distributed evenly throughout the PSD. We find here that PSD-93 has a nearly identical distribution suggesting that PSD-95 and PSD-93 could perform similar roles. SAP97, in contrast, is concentrated near edges of cleft sides of the PSDs, and in small clumps on their cytoplasmic sides. The homogenous distribution of PSD-95 and PSD-93 throughout the PSD is consistent with their being part of a backbone that stabilizes their various binding partners within the PSD. The distribution of SAP97 confirms that this protein is actually an integral component of the PSD, and suggests that it may have a role in inserting or stabilizing its main binding partner, Glu-R1, at the edge of the PSD.

Original languageEnglish (US)
Pages (from-to)239-250
Number of pages12
JournalBrain Cell Biology
Volume35
Issue number4-6
DOIs
Publication statusPublished - Dec 2006
Externally publishedYes

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ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Cell Biology

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