Distribution of radiolabeled endotoxin with particular reference to the eye: Concise communication

James (Jim) Rosenbaum, P. A. Hendricks, J. E. Shively, I. R. McDougall

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

A single systemic injection of endotoxin (lipopolysaccharide or LPS) reproducibly induces a cellular infiltrate in the uveal tract of the rat eye within 24 hr. Other organs are not comparably sensitive to systemic endotoxin. One hypothesis to explain this unique sensitivity is that endotoxin is preferentially bound by ocular tissue. We tested this hypothesis by studying the distribution in the rat of intravenously injected endotoxin that had been radiolabeled with Tc-99m or P-32. With either radionuclide the concentration of endotoxin per gram of tissue at a variety of times after injection ranging from 5 min to 3 hr and 45 min, was markedly less in the eye than in the liver, kidney, or spleen. A study with radiolabeled jalbumin indicated that these differences could not be ascribed solely to the organ's blood volume. They demonstrate, therefore, that the eye does not preferentially bind endotoxin, and they are compatible with the hypothesis that endotoxin's ocular effects are indirectly mediated.

Original languageEnglish (US)
Pages (from-to)29-33
Number of pages5
JournalJournal of Nuclear Medicine
Volume24
Issue number1
StatePublished - 1983
Externally publishedYes

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Endotoxins
Communication
Injections
Organ Size
Blood Volume
Radioisotopes
Lipopolysaccharides
Spleen
Kidney
Liver

ASJC Scopus subject areas

  • Radiological and Ultrasound Technology

Cite this

Distribution of radiolabeled endotoxin with particular reference to the eye : Concise communication. / Rosenbaum, James (Jim); Hendricks, P. A.; Shively, J. E.; McDougall, I. R.

In: Journal of Nuclear Medicine, Vol. 24, No. 1, 1983, p. 29-33.

Research output: Contribution to journalArticle

Rosenbaum, James (Jim) ; Hendricks, P. A. ; Shively, J. E. ; McDougall, I. R. / Distribution of radiolabeled endotoxin with particular reference to the eye : Concise communication. In: Journal of Nuclear Medicine. 1983 ; Vol. 24, No. 1. pp. 29-33.
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