Distribution and regulation of aromatase activity in the rat hypothalamus and limbic system

Charles E. Roselli; Linda E. Horton; John A. Resko; Charles E. Roselli; Linda E. Horton; John A. Resko

doi:10.1210/endo-117-6-2471

Distribution and regulation of aromatase activity in the rat hypothalamus and limbic system

Charles E. Roselli, Linda E. Horton, John A. Resko, Charles E. Roselli, Linda E. Horton, John A. Resko

Chemical Physiology and Biochemistry

Research output: Contribution to journal › Article › peer-review

405 Scopus citations

Abstract

Conversion of androgen to estrogen in the rat brain is catalyzed by aromatase enzymes. The maximum concentrations of these enzymes are found within the hypothalamus and amygdala, where they appear to play an important role in the process by which androgens affect both behavior and neuroendocrine function. In the present study, we measured the levels of aromatase activity (AA) in 20 nuclei and brain regions of the adult rat brain. Individual nuclei were microdissected from 600-μm frozen sections. Tissues from 3 animals were pooled, and AA was measured by an in vitro radiometric assay that quantifies the stereospecific production of ³H₂O from [1β-³H]androstenedione as an index of estrogen formation. Our results provide a quantitative profile of AA in specific hypothalamic and limbic nuclei of the rat brain as well as information on the control of AA within these discrete regions.

Original language	English (US)
Pages (from-to)	2471-2477
Number of pages	7
Journal	Endocrinology
Volume	117
Issue number	6
DOIs	https://doi.org/10.1210/endo-117-6-2471
State	Published - 1985

ASJC Scopus subject areas

Endocrinology

Access to Document

10.1210/endo-117-6-2471

Cite this

@article{f46683641e6d4552819f9b7cf0a51e7a,

title = "Distribution and regulation of aromatase activity in the rat hypothalamus and limbic system",

abstract = "Conversion of androgen to estrogen in the rat brain is catalyzed by aromatase enzymes. The maximum concentrations of these enzymes are found within the hypothalamus and amygdala, where they appear to play an important role in the process by which androgens affect both behavior and neuroendocrine function. In the present study, we measured the levels of aromatase activity (AA) in 20 nuclei and brain regions of the adult rat brain. Individual nuclei were microdissected from 600-μm frozen sections. Tissues from 3 animals were pooled, and AA was measured by an in vitro radiometric assay that quantifies the stereospecific production of 3H2O from [1β-3H]androstenedione as an index of estrogen formation. Our results provide a quantitative profile of AA in specific hypothalamic and limbic nuclei of the rat brain as well as information on the control of AA within these discrete regions.",

author = "Roselli, {Charles E.} and Horton, {Linda E.} and Resko, {John A.} and Roselli, {Charles E.} and Horton, {Linda E.} and Resko, {John A.}",

year = "1985",

doi = "10.1210/endo-117-6-2471",

language = "English (US)",

volume = "117",

pages = "2471--2477",

journal = "Endocrinology",

issn = "0013-7227",

publisher = "The Endocrine Society",

number = "6",

}

TY - JOUR

T1 - Distribution and regulation of aromatase activity in the rat hypothalamus and limbic system

AU - Roselli, Charles E.

AU - Horton, Linda E.

AU - Resko, John A.

AU - Roselli, Charles E.

AU - Horton, Linda E.

AU - Resko, John A.

PY - 1985

Y1 - 1985

N2 - Conversion of androgen to estrogen in the rat brain is catalyzed by aromatase enzymes. The maximum concentrations of these enzymes are found within the hypothalamus and amygdala, where they appear to play an important role in the process by which androgens affect both behavior and neuroendocrine function. In the present study, we measured the levels of aromatase activity (AA) in 20 nuclei and brain regions of the adult rat brain. Individual nuclei were microdissected from 600-μm frozen sections. Tissues from 3 animals were pooled, and AA was measured by an in vitro radiometric assay that quantifies the stereospecific production of 3H2O from [1β-3H]androstenedione as an index of estrogen formation. Our results provide a quantitative profile of AA in specific hypothalamic and limbic nuclei of the rat brain as well as information on the control of AA within these discrete regions.

AB - Conversion of androgen to estrogen in the rat brain is catalyzed by aromatase enzymes. The maximum concentrations of these enzymes are found within the hypothalamus and amygdala, where they appear to play an important role in the process by which androgens affect both behavior and neuroendocrine function. In the present study, we measured the levels of aromatase activity (AA) in 20 nuclei and brain regions of the adult rat brain. Individual nuclei were microdissected from 600-μm frozen sections. Tissues from 3 animals were pooled, and AA was measured by an in vitro radiometric assay that quantifies the stereospecific production of 3H2O from [1β-3H]androstenedione as an index of estrogen formation. Our results provide a quantitative profile of AA in specific hypothalamic and limbic nuclei of the rat brain as well as information on the control of AA within these discrete regions.

UR - http://www.scopus.com/inward/record.url?scp=0022348964&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022348964&partnerID=8YFLogxK

U2 - 10.1210/endo-117-6-2471

DO - 10.1210/endo-117-6-2471

M3 - Article

C2 - 4065042

AN - SCOPUS:0022348964

SN - 0013-7227

VL - 117

SP - 2471

EP - 2477

JO - Endocrinology

JF - Endocrinology

IS - 6

ER -

Distribution and regulation of aromatase activity in the rat hypothalamus and limbic system

Abstract

ASJC Scopus subject areas

Access to Document

Other files and links

Fingerprint

Cite this