Distal regulatory functions for the uvrC of E. coli

Surendra Sharma, Thomas Stark, Robb Moses

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

We find that the uvrC gene is preceded by three promoters (P1, P2 and P3), identified by heparin-resistant R1'lA polymerase-DNA complex formation. P2 and P3 promoters are located proximal to the 5' end of the uvrC gene, while the P1 promoter is separated from the uvrC structural gene by an interposed DNA region of more than 1 kb. We have reported that P2 and P3 are not sufficient to promote uvrC complementation. However, plasmids containing the direct fusion of the P1 promoter to the uvrC gene complements the uvrC defect. Insertion of IS1 downstream from the P1 promoter leads to efficient synthesis of the uvrC protein as measured in maxicells. Fusion of the lac promoter to the uvrC structural gene can substitute for in vivo regulatory functions. We conclude that uvrC protein synthesis is controlled in a complex manner and that a distal promoter, P1, is required.

Original languageEnglish (US)
Pages (from-to)5341-5354
Number of pages14
JournalNucleic Acids Research
Volume12
Issue number13
DOIs
StatePublished - Jul 11 1984
Externally publishedYes

Fingerprint

Promoter
Escherichia coli
Escherichia Coli
Genes
Gene
DNA
Fusion reactions
Proteins
Fusion
DNA-Directed DNA Polymerase
Heparin
Complementation
Protein Synthesis
Plasmids
Substitute
Insertion
Defects
Complement
Synthesis
Sufficient

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Distal regulatory functions for the uvrC of E. coli. / Sharma, Surendra; Stark, Thomas; Moses, Robb.

In: Nucleic Acids Research, Vol. 12, No. 13, 11.07.1984, p. 5341-5354.

Research output: Contribution to journalArticle

Sharma, Surendra ; Stark, Thomas ; Moses, Robb. / Distal regulatory functions for the uvrC of E. coli. In: Nucleic Acids Research. 1984 ; Vol. 12, No. 13. pp. 5341-5354.
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