Disruption of CUL3-mediated ubiquitination causes proximal tubule injury and kidney fibrosis

Turgay Saritas, Catherina A. Cuevas, Mohammed Z. Ferdaus, Christoph Kuppe, Rafael Kramann, Marcus J. Moeller, Jürgen Floege, Jeffrey D. Singer, James (Jim) McCormick

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cullin 3 (CUL3) is part of the ubiquitin proteasomal system and controls several cellular processes critical for normal organ function including the cell cycle, and Keap1/Nrf2 signaling. Kidney tubule-specific Cul3 disruption causes tubulointerstitial fibrosis, but little is known about the mechanisms. Therefore, we tested the hypothesis that dysregulation of the cell cycle and Keap1/Nrf2 pathway play a role in initiating the kidney injury upon Cul3 disruption. Cul3 deletion increased expression of cyclin E and p21, associated with uncontrolled proliferation, DNA damage, and apoptosis, all of which preceded proximal tubule injury. The cdk2-cyclin E inhibitor roscovitine did not prevent the effects of Cul3 deletion, but instead exacerbated the kidney injury. Injury occurred despite accumulation and activation of CUL3 substrate Keap1/Nrf2, proposed to be protective in kidney injury. Cul3 disruption led to progressive interstitial inflammation, functionally relevant renal fibrosis and death. Finally, we observed reduced CUL3 expression in several AKI and CKD mouse models and in fibrotic human kidney tissue. These data establish CUL3 knockout mice as a novel genetic CKD model in which dysregulation of the cell cycle may play a primary role in initiating tubule injury, and that CUL3 dysregulation could contribute to acute and fibrotic kidney disease.

Original languageEnglish (US)
Article number4596
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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Cullin Proteins
Proximal Kidney Tubule
Ubiquitination
Fibrosis
Kidney
Wounds and Injuries
Cyclin E
Cell Cycle
Kidney Tubules
Genetic Models
Kidney Diseases
Ubiquitin
Knockout Mice
DNA Damage
Apoptosis
Inflammation

ASJC Scopus subject areas

  • General

Cite this

Disruption of CUL3-mediated ubiquitination causes proximal tubule injury and kidney fibrosis. / Saritas, Turgay; Cuevas, Catherina A.; Ferdaus, Mohammed Z.; Kuppe, Christoph; Kramann, Rafael; Moeller, Marcus J.; Floege, Jürgen; Singer, Jeffrey D.; McCormick, James (Jim).

In: Scientific Reports, Vol. 9, No. 1, 4596, 01.12.2019.

Research output: Contribution to journalArticle

Saritas, T, Cuevas, CA, Ferdaus, MZ, Kuppe, C, Kramann, R, Moeller, MJ, Floege, J, Singer, JD & McCormick, JJ 2019, 'Disruption of CUL3-mediated ubiquitination causes proximal tubule injury and kidney fibrosis', Scientific Reports, vol. 9, no. 1, 4596. https://doi.org/10.1038/s41598-019-40795-0
Saritas, Turgay ; Cuevas, Catherina A. ; Ferdaus, Mohammed Z. ; Kuppe, Christoph ; Kramann, Rafael ; Moeller, Marcus J. ; Floege, Jürgen ; Singer, Jeffrey D. ; McCormick, James (Jim). / Disruption of CUL3-mediated ubiquitination causes proximal tubule injury and kidney fibrosis. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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