Discriminative stimulus effects of ethanol in rats using a three-choice ethanol-midazolam-water discrimination

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Three-choice discrimination procedures are used to characterize how similar the discriminative stimulus effects of two drugs are in relation to each other. This procedure has suggested similarities between ethanol and ligands that positively modulate the γ-aminobutyric acid type A (GABAA) receptor complex. As an extension to these studies, male Long-Evans rats were trained to discriminate midazolam (3.0 mg/kg, i.p.) from ethanol (1.0 g/kg, i.g.) from water (2.3 ml, i.g.) in a three-lever, food reinforced task. Substitution tests were conducted following administration of GABA A-positive modulators, noncompetitive N-methyl-D-aspartate (NMDA) antagonists, 5-HT1B agonists and isopropanol. Among the GABA A-positive modulators, diazepam was the only drug that completely substituted for midazolam; both pentobarbital and the neurosteroid allopregnanolone showed partial midazolam substitution. The NMDA antagonist dizocilpine substituted for ethanol, while phencyclidine showed no substitution for either ethanol or midazolam. The serotonin agonists tested also showed no substitution for either ethanol or midazolam. Isopropanol was the only other drug that completely substituted for ethanol. These data extend previous findings from an ethanol-pentobarbital-water discrimination and further define training conditions that result in a conditional basis for the ethanol discrimination where only those drugs with pharmacological heterogeneous effects similar to ethanol produce a full ethanol-like effect.

Original languageEnglish (US)
Pages (from-to)555-567
Number of pages13
JournalBehavioural Pharmacology
Volume15
Issue number8
DOIs
StatePublished - Dec 2004
Externally publishedYes

Fingerprint

Midazolam
Ethanol
Water
2-Propanol
Pentobarbital
N-Methylaspartate
Pharmaceutical Preparations
gamma-Aminobutyric Acid
Pregnanolone
Serotonin 5-HT1 Receptor Agonists
Aminobutyrates
Phencyclidine
Serotonin Receptor Agonists
Long Evans Rats
Dizocilpine Maleate
GABA-A Receptors
Diazepam
Neurotransmitter Agents
Pharmacology
Ligands

Keywords

  • 5-HT agonists
  • Alcohol
  • Drug discrimination
  • Ethanol
  • GABA-positive modulators
  • Midazolam
  • NMDA antagonists
  • Rat

ASJC Scopus subject areas

  • Pharmacology
  • Neuroscience(all)

Cite this

Discriminative stimulus effects of ethanol in rats using a three-choice ethanol-midazolam-water discrimination. / Porcu, P.; Grant, Kathleen (Kathy).

In: Behavioural Pharmacology, Vol. 15, No. 8, 12.2004, p. 555-567.

Research output: Contribution to journalArticle

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