Direct-repeat analysis of chromatid interactions during intrachromosomal recombination in mouse cells

Roni J. Bollag, Robert (Mike) Liskay

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Homologous intrachromosomal recombination between linked genes can involve interactions that are either intramolecular (intrachromatid) or intermolecular (sister chromatid). To assess the relative proportions of chromatid interactions, we report studies of intrachromosomal recombination in mouse L cells containing herpes simplex virus thymidine kinase genes in two alternative configurations of direct repeats. By comparing products of reciprocal exchanges between these two configurations, we conclude that the majority of interactions that give rise to crossover products involve unequally paired sister chromatids after DNA replication. Analyses of an additional class of crossover products that involve discontinuous associated gene conversion suggest that these recombination events involve a heteroduplex DNA intermediate.

Original languageEnglish (US)
Pages (from-to)4839-4845
Number of pages7
JournalMolecular and Cellular Biology
Volume11
Issue number10
StatePublished - 1991
Externally publishedYes

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Chromatids
Nucleic Acid Repetitive Sequences
Genetic Recombination
Nucleic Acid Heteroduplexes
Gene Conversion
Thymidine Kinase
Homologous Recombination
Simplexvirus
DNA Replication
Genes

ASJC Scopus subject areas

  • Cell Biology
  • Genetics
  • Molecular Biology

Cite this

Direct-repeat analysis of chromatid interactions during intrachromosomal recombination in mouse cells. / Bollag, Roni J.; Liskay, Robert (Mike).

In: Molecular and Cellular Biology, Vol. 11, No. 10, 1991, p. 4839-4845.

Research output: Contribution to journalArticle

Bollag, Roni J. ; Liskay, Robert (Mike). / Direct-repeat analysis of chromatid interactions during intrachromosomal recombination in mouse cells. In: Molecular and Cellular Biology. 1991 ; Vol. 11, No. 10. pp. 4839-4845.
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