Direct observation of kinesin stepping by optical trapping interferometry

Karel Svoboda, Christoph F. Schmidt, Bruce J. Schnapp, Steven M. Block

Research output: Contribution to journalArticle

1461 Scopus citations

Abstract

Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

Original languageEnglish (US)
Pages (from-to)721-727
Number of pages7
JournalNature
Volume365
Issue number6448
DOIs
StatePublished - Jan 1 1993

ASJC Scopus subject areas

  • General

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