Direct observation of kinesin stepping by optical trapping interferometry

Karel Svoboda, Christoph F. Schmidt, Bruce Schnapp, Steven M. Block

Research output: Contribution to journalArticle

1445 Citations (Scopus)

Abstract

Do biological motors move with regular steps? To address this question, we constructed instrumentation with the spatial and temporal sensitivity to resolve movement on a molecular scale. We deposited silica beads carrying single molecules of the motor protein kinesin on microtubules using optical tweezers and analysed their motion under controlled loads by interferometry. We find that kinesin moves with 8-nm steps.

Original languageEnglish (US)
Pages (from-to)721-727
Number of pages7
JournalNature
Volume365
Issue number6448
StatePublished - Oct 21 1993
Externally publishedYes

Fingerprint

Optical Tweezers
Interferometry
Kinesin
Observation
Microtubules
Silicon Dioxide
Proteins

ASJC Scopus subject areas

  • General

Cite this

Svoboda, K., Schmidt, C. F., Schnapp, B., & Block, S. M. (1993). Direct observation of kinesin stepping by optical trapping interferometry. Nature, 365(6448), 721-727.

Direct observation of kinesin stepping by optical trapping interferometry. / Svoboda, Karel; Schmidt, Christoph F.; Schnapp, Bruce; Block, Steven M.

In: Nature, Vol. 365, No. 6448, 21.10.1993, p. 721-727.

Research output: Contribution to journalArticle

Svoboda, K, Schmidt, CF, Schnapp, B & Block, SM 1993, 'Direct observation of kinesin stepping by optical trapping interferometry', Nature, vol. 365, no. 6448, pp. 721-727.
Svoboda K, Schmidt CF, Schnapp B, Block SM. Direct observation of kinesin stepping by optical trapping interferometry. Nature. 1993 Oct 21;365(6448):721-727.
Svoboda, Karel ; Schmidt, Christoph F. ; Schnapp, Bruce ; Block, Steven M. / Direct observation of kinesin stepping by optical trapping interferometry. In: Nature. 1993 ; Vol. 365, No. 6448. pp. 721-727.
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