Differential microRNA profile underlies the divergent healing responses in skin and oral mucosal wounds

Alyne Simões, Lin Chen, Zujian Chen, Yan Zhao, Shang Gao, Phillip Marucha, Yang Dai, Luisa A. DiPietro, Xiaofeng Zhou

Research output: Contribution to journalArticle

Abstract

Oral mucosal wounds heal faster than skin wounds, yet the role of microRNAs in this differential healing has never been examined. To delineate the role of microRNAs in this site-specific injury response, we first compared the microRNAome of uninjured skin and oral mucosa in mice. A total of 53 tissue-specific microRNAs for skin and oral mucosa epithelium were identified. The most striking difference was the high abundance of miR-10a/b in skin (accounting for 21.10% of the skin microRNAome) as compared to their low expression in oral mucosa (2.87%). We further examined the dynamic changes of microRNAome throughout the time course of skin and oral mucosal wound healing. More differentially expressed microRNAs were identified in skin wounds than oral wounds (200 and 33, respectively). More specifically, miR-10a/b was significantly down-regulated in skin but not oral wounds. In contrast, up-regulation of miR-21 was observed in both skin and oral wounds. The therapeutic potential of miR-10b and miR-21 in accelerating wound closure was demonstrated in in vitro assays and in a murine skin wound model. Thus, we provided the first site-specific microRNA profile of skin and oral mucosal wound healing, and demonstrate the feasibility of a microRNA-based therapy for promoting wound closure.

Original languageEnglish (US)
Article number7160
JournalScientific Reports
Volume9
Issue number1
DOIs
StatePublished - Dec 1 2019

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MicroRNAs
Skin
Wounds and Injuries
Mouth Mucosa
Wound Healing
Up-Regulation
Epithelium

ASJC Scopus subject areas

  • General

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Differential microRNA profile underlies the divergent healing responses in skin and oral mucosal wounds. / Simões, Alyne; Chen, Lin; Chen, Zujian; Zhao, Yan; Gao, Shang; Marucha, Phillip; Dai, Yang; DiPietro, Luisa A.; Zhou, Xiaofeng.

In: Scientific Reports, Vol. 9, No. 1, 7160, 01.12.2019.

Research output: Contribution to journalArticle

Simões, Alyne ; Chen, Lin ; Chen, Zujian ; Zhao, Yan ; Gao, Shang ; Marucha, Phillip ; Dai, Yang ; DiPietro, Luisa A. ; Zhou, Xiaofeng. / Differential microRNA profile underlies the divergent healing responses in skin and oral mucosal wounds. In: Scientific Reports. 2019 ; Vol. 9, No. 1.
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abstract = "Oral mucosal wounds heal faster than skin wounds, yet the role of microRNAs in this differential healing has never been examined. To delineate the role of microRNAs in this site-specific injury response, we first compared the microRNAome of uninjured skin and oral mucosa in mice. A total of 53 tissue-specific microRNAs for skin and oral mucosa epithelium were identified. The most striking difference was the high abundance of miR-10a/b in skin (accounting for 21.10{\%} of the skin microRNAome) as compared to their low expression in oral mucosa (2.87{\%}). We further examined the dynamic changes of microRNAome throughout the time course of skin and oral mucosal wound healing. More differentially expressed microRNAs were identified in skin wounds than oral wounds (200 and 33, respectively). More specifically, miR-10a/b was significantly down-regulated in skin but not oral wounds. In contrast, up-regulation of miR-21 was observed in both skin and oral wounds. The therapeutic potential of miR-10b and miR-21 in accelerating wound closure was demonstrated in in vitro assays and in a murine skin wound model. Thus, we provided the first site-specific microRNA profile of skin and oral mucosal wound healing, and demonstrate the feasibility of a microRNA-based therapy for promoting wound closure.",
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