Differential maturation of murine bone-marrow derived dendritic cells with lipopolysaccharide and tumor necrosis factor-α

Philip A. Efron, Hironori Tsujimoto, Frances Bahjat, Ricardo Ungaro, Justin Debernardis, Cynthia Tannahill, Henry V. Baker, Carl K. Edwards, Lyle L. Moldawer

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Dendritic cells (DCs) play a key role in the interface between the innate and acquired immune systems. In response to both exogenous as well as endogenous signals, DCs undergo a programmed maturation to become an efficient, antigen-presenting cell. Yet little is known regarding the differential responses by endogenous versus exogenous stimuli on DC maturation. In the present report, we have compared the phenotypic, functional, and genome-wide expression responses associated with maturation by bone marrow derived DCs to either an endogenous danger signal, tumor necrosis factor-α (TNF-α), or a microbial product, bacterial lipopolysaccharide (LPS). Examination of the cell surface expression of DCs as well as cytokine production demonstrated that patterns of DC maturation varied dramatically depending upon the stimulus. Whereas LPS was highly effective in terms of inducing phenotypic and functional maturation, TNF-α exposure produced a phenotypically distinct DC. Gene expression patterns in DCs 6 and 24 h after LPS and TNF-α exposure revealed that these activation signals produce fundamentally different genomic responses. Supervised analysis revealed that the expression of 929 probe sets discriminated among the treatment groups, and the patterns of gene expression in TNF-α stimulated DCs were more similar to unstimulated cells at both 6 and 24 h post-stimulation than to LPS-stimulated cells at the same time points. These findings reveal that DCs are capable of a varying phenotypic response to different antigens and endogenous signals.

Original languageEnglish (US)
Pages (from-to)145-160
Number of pages16
JournalJournal of Endotoxin Research
Volume11
Issue number3
DOIs
StatePublished - 2005
Externally publishedYes

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Dendritic Cells
Lipopolysaccharides
Bone
Tumor Necrosis Factor-alpha
Bone Marrow
Gene expression
Gene Expression
Immune system
Antigen-Presenting Cells
Immune System
Genes
Chemical activation
Genome
Cytokines
Antigens

Keywords

  • Cytokines
  • Gene expression analysis
  • Inflammation

ASJC Scopus subject areas

  • Microbiology
  • Immunology
  • Toxicology

Cite this

Efron, P. A., Tsujimoto, H., Bahjat, F., Ungaro, R., Debernardis, J., Tannahill, C., ... Moldawer, L. L. (2005). Differential maturation of murine bone-marrow derived dendritic cells with lipopolysaccharide and tumor necrosis factor-α. Journal of Endotoxin Research, 11(3), 145-160. https://doi.org/10.1179/096805105X46583

Differential maturation of murine bone-marrow derived dendritic cells with lipopolysaccharide and tumor necrosis factor-α. / Efron, Philip A.; Tsujimoto, Hironori; Bahjat, Frances; Ungaro, Ricardo; Debernardis, Justin; Tannahill, Cynthia; Baker, Henry V.; Edwards, Carl K.; Moldawer, Lyle L.

In: Journal of Endotoxin Research, Vol. 11, No. 3, 2005, p. 145-160.

Research output: Contribution to journalArticle

Efron, PA, Tsujimoto, H, Bahjat, F, Ungaro, R, Debernardis, J, Tannahill, C, Baker, HV, Edwards, CK & Moldawer, LL 2005, 'Differential maturation of murine bone-marrow derived dendritic cells with lipopolysaccharide and tumor necrosis factor-α', Journal of Endotoxin Research, vol. 11, no. 3, pp. 145-160. https://doi.org/10.1179/096805105X46583
Efron, Philip A. ; Tsujimoto, Hironori ; Bahjat, Frances ; Ungaro, Ricardo ; Debernardis, Justin ; Tannahill, Cynthia ; Baker, Henry V. ; Edwards, Carl K. ; Moldawer, Lyle L. / Differential maturation of murine bone-marrow derived dendritic cells with lipopolysaccharide and tumor necrosis factor-α. In: Journal of Endotoxin Research. 2005 ; Vol. 11, No. 3. pp. 145-160.
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