Differential expression of lymphocyte homing receptors by human memory/effector T cells in pulmonary versus cutaneous immune effector sites

Louis Picker, Richard J. Martin, Anne Trumble, Lee S. Newman, Patricia A. Collins, Paul R. Bergstresser, Donald Y M Leung

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

The heterogeneous expression of lymphocyte homing receptors (HR) by the (CD45RA(low)/RO(high)) memory/effector T cell population in the human is thought to define subsets with tissue-selective recirculatory potential. To investigate further the localization characteristics of these T cells, we used multiparameter flow cytometry to quantitate T cell subsets defined by expression of the skin-selective HR called the cutaneous lymphocyte-associated antigen (CLA), the peripheral lymph node (PLN) HR L-selectin, the mucosal-associated HR α4β7-integrin, and the mucosal-associated adhesion molecule αeβ7-integrin in either cutaneous or pulmonary immune effector sites and corresponding peripheral blood. Compared to peripheral blood, skin T cells were highly enriched for the CLA+/L-selectin+/αeβ7-integrin- memory/effector subset, whereas lung memory/effector T cells were predominantly CLA(- to low) L-selectin-, and almost half were αeβ7-integrin+. α4β7-integrin expressing memory/effector T cells were diminished in both skin and lung, suggesting that this HR is not a major participant in determining localization specificity in either of these sites. The characteristic pulmonary T cell HR phenotype did not significantly differ between the normal subjects and those with pulmonary inflammatory disease, and did not correlate with markers of T cell activation. Induction of a rapid up-regulation of pulmonary inflammation via intrabronchial allergen challenge in asthmatic patients tended to decrease localization specificity, resulting in a more general importation of memory/effector subsets. Taken together, these results suggest that tissue microenvironments play a major role in determining the character of local T cell infiltrates via their ability to import and retain memory/effector subsets selectively or, more generally, depending on the intensity of local inflammatory stimuli.

Original languageEnglish (US)
Pages (from-to)1269-1277
Number of pages9
JournalEuropean Journal of Immunology
Volume24
Issue number6
DOIs
StatePublished - Jun 1994
Externally publishedYes

Fingerprint

Lymphocyte Homing Receptors
Integrins
T-Lymphocytes
Lung
Skin
L-Selectin
Lymphocytes
Antigens
Aptitude
T-Lymphocyte Subsets
Allergens
Lung Diseases
Pneumonia
Flow Cytometry
Up-Regulation
Phenotype

Keywords

  • Homing receptors
  • Lymphocyte extravasation
  • Memory-effector T cell subsets

ASJC Scopus subject areas

  • Immunology

Cite this

Differential expression of lymphocyte homing receptors by human memory/effector T cells in pulmonary versus cutaneous immune effector sites. / Picker, Louis; Martin, Richard J.; Trumble, Anne; Newman, Lee S.; Collins, Patricia A.; Bergstresser, Paul R.; Leung, Donald Y M.

In: European Journal of Immunology, Vol. 24, No. 6, 06.1994, p. 1269-1277.

Research output: Contribution to journalArticle

Picker, Louis ; Martin, Richard J. ; Trumble, Anne ; Newman, Lee S. ; Collins, Patricia A. ; Bergstresser, Paul R. ; Leung, Donald Y M. / Differential expression of lymphocyte homing receptors by human memory/effector T cells in pulmonary versus cutaneous immune effector sites. In: European Journal of Immunology. 1994 ; Vol. 24, No. 6. pp. 1269-1277.
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