Dietary cholesterol feeding suppresses human cholesterol synthesis measured by deuterium incorporation and urinary mevalonic acid levels

Peter J H Jones, Anuradha Pappu, Lauren Hatcher, Zi Chi Li, D. Roger Illingworth, William E. Connor

Research output: Contribution to journalArticle

62 Citations (Scopus)

Abstract

The objective of this study was to measure the response of cholesterol biosynthesis in subjects to three different amounts of dietary cholesterol: 50 (low), 350 (medium), and 650 (high) mg cholesterol per 2800 kcal. Individuals with low (n =7), normal (n =12), and elevated (n= 11) plasma cholesterol concentrations consumed in random order solid food test diets (15%, 55%, and 30% of energy as protein, carbohydrate, and fat, respectively) at each dietary cholesterol level. The three diets were consumed for 4 weeks each, and each dietary phase was separated by a 4-week washout period. During the final week of each diet, 0.7 g D 2O was given per kilogram of body water and deuterium incorporation into the erythrocyte cholesterol pool was measured for 24 hours. Urinary mevalonate levels were also determined in samples obtained during two consecutive 24 hour periods. Both techniques provided measurements of whole-body cholesterol biosynthesis. In all subjects the cholesterol synthesis rate as measured by deuterium incorporation was significantly lower (P

Original languageEnglish (US)
Pages (from-to)1222-1228
Number of pages7
JournalArteriosclerosis, Thrombosis, and Vascular Biology
Volume16
Issue number10
StatePublished - 1996

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Dietary Cholesterol
Mevalonic Acid
Deuterium
Cholesterol
Diet
Body Water
Erythrocytes
Fats
Carbohydrates
Food
Proteins

Keywords

  • cholesterol synthesis
  • deuterium
  • dietary cholesterol
  • lipoproteins
  • mevalonic acid

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Dietary cholesterol feeding suppresses human cholesterol synthesis measured by deuterium incorporation and urinary mevalonic acid levels. / Jones, Peter J H; Pappu, Anuradha; Hatcher, Lauren; Li, Zi Chi; Illingworth, D. Roger; Connor, William E.

In: Arteriosclerosis, Thrombosis, and Vascular Biology, Vol. 16, No. 10, 1996, p. 1222-1228.

Research output: Contribution to journalArticle

Jones, Peter J H ; Pappu, Anuradha ; Hatcher, Lauren ; Li, Zi Chi ; Illingworth, D. Roger ; Connor, William E. / Dietary cholesterol feeding suppresses human cholesterol synthesis measured by deuterium incorporation and urinary mevalonic acid levels. In: Arteriosclerosis, Thrombosis, and Vascular Biology. 1996 ; Vol. 16, No. 10. pp. 1222-1228.
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