Diagnostic value of rectal suction biopsies using calretinin immunohistochemical staining in Hirschsprung's disease

Viet Quoc Tran, Kim Thien Lam, Dinh Quang Truong, Minh Hoang Dang, Thao Thi Phuong Doan, Valerie Segers, Marilyn Butler, Annie Robert, Philippe Goyens, Henri Steyaert

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background The study investigates the diagnostic value of calretinin immunohistochemical staining (CIS) on rectal suction biopsies (RSB) in Hirschsprung's disease (HD). Methods A prospective study was conducted at Children's Hospital 2 in Ho Chi Minh City, Vietnam, from January through December 2015. Patients suspected of HD during this period underwent RSB and were followed in order to assess the accuracy of the diagnostic test with CIS compared with conventional histology (H&E). Results A total of 188 children with RSB were investigated. Median age was 7.1 (range 0.2–159) months with 65.4% boys. HD was confirmed in 80 (42.6%) children. There were 1 false positive and no false-negative cases. The sensitivity and specificity were 100% (80/80) and 99.1% (107/108) for CIS and 100% and 85.2% for H&E, respectively. Cohen's kappa coefficient was 0.9891 with a diagnostic accuracy of 99.5% for CIS, compared with 0.8303 and 91.5% for H&E, respectively. There were no serious complications related to the RSB. Conclusion RSB with CIS is a useful diagnostic method for HD, with easy interpretation and no need for cryostat. CIS has a high diagnostic accuracy and should be considered as the primary method for the diagnosis of HD by RSB. Level of evidence: Diagnostic Studies – Level I.

Original languageEnglish (US)
Pages (from-to)2005-2009
Number of pages5
JournalJournal of Pediatric Surgery
Volume51
Issue number12
DOIs
StatePublished - Dec 1 2016

Fingerprint

Calbindin 2
Hirschsprung Disease
Suction
Staining and Labeling
Biopsy
Vietnam
Routine Diagnostic Tests
Histology
Prospective Studies
Sensitivity and Specificity

Keywords

  • Acetyl cholinesterase
  • Calretinin immunochemistry
  • Calretinin immunohistochemical staining
  • Hematoxylin & eosin
  • Hirschsprung's disease
  • Rectal suction biopsy

ASJC Scopus subject areas

  • Pediatrics, Perinatology, and Child Health
  • Surgery

Cite this

Tran, V. Q., Lam, K. T., Truong, D. Q., Dang, M. H., Doan, T. T. P., Segers, V., ... Steyaert, H. (2016). Diagnostic value of rectal suction biopsies using calretinin immunohistochemical staining in Hirschsprung's disease. Journal of Pediatric Surgery, 51(12), 2005-2009. https://doi.org/10.1016/j.jpedsurg.2016.09.027

Diagnostic value of rectal suction biopsies using calretinin immunohistochemical staining in Hirschsprung's disease. / Tran, Viet Quoc; Lam, Kim Thien; Truong, Dinh Quang; Dang, Minh Hoang; Doan, Thao Thi Phuong; Segers, Valerie; Butler, Marilyn; Robert, Annie; Goyens, Philippe; Steyaert, Henri.

In: Journal of Pediatric Surgery, Vol. 51, No. 12, 01.12.2016, p. 2005-2009.

Research output: Contribution to journalArticle

Tran, VQ, Lam, KT, Truong, DQ, Dang, MH, Doan, TTP, Segers, V, Butler, M, Robert, A, Goyens, P & Steyaert, H 2016, 'Diagnostic value of rectal suction biopsies using calretinin immunohistochemical staining in Hirschsprung's disease', Journal of Pediatric Surgery, vol. 51, no. 12, pp. 2005-2009. https://doi.org/10.1016/j.jpedsurg.2016.09.027
Tran, Viet Quoc ; Lam, Kim Thien ; Truong, Dinh Quang ; Dang, Minh Hoang ; Doan, Thao Thi Phuong ; Segers, Valerie ; Butler, Marilyn ; Robert, Annie ; Goyens, Philippe ; Steyaert, Henri. / Diagnostic value of rectal suction biopsies using calretinin immunohistochemical staining in Hirschsprung's disease. In: Journal of Pediatric Surgery. 2016 ; Vol. 51, No. 12. pp. 2005-2009.
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abstract = "Background The study investigates the diagnostic value of calretinin immunohistochemical staining (CIS) on rectal suction biopsies (RSB) in Hirschsprung's disease (HD). Methods A prospective study was conducted at Children's Hospital 2 in Ho Chi Minh City, Vietnam, from January through December 2015. Patients suspected of HD during this period underwent RSB and were followed in order to assess the accuracy of the diagnostic test with CIS compared with conventional histology (H&E). Results A total of 188 children with RSB were investigated. Median age was 7.1 (range 0.2–159) months with 65.4{\%} boys. HD was confirmed in 80 (42.6{\%}) children. There were 1 false positive and no false-negative cases. The sensitivity and specificity were 100{\%} (80/80) and 99.1{\%} (107/108) for CIS and 100{\%} and 85.2{\%} for H&E, respectively. Cohen's kappa coefficient was 0.9891 with a diagnostic accuracy of 99.5{\%} for CIS, compared with 0.8303 and 91.5{\%} for H&E, respectively. There were no serious complications related to the RSB. Conclusion RSB with CIS is a useful diagnostic method for HD, with easy interpretation and no need for cryostat. CIS has a high diagnostic accuracy and should be considered as the primary method for the diagnosis of HD by RSB. Level of evidence: Diagnostic Studies – Level I.",
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AU - Tran, Viet Quoc

AU - Lam, Kim Thien

AU - Truong, Dinh Quang

AU - Dang, Minh Hoang

AU - Doan, Thao Thi Phuong

AU - Segers, Valerie

AU - Butler, Marilyn

AU - Robert, Annie

AU - Goyens, Philippe

AU - Steyaert, Henri

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N2 - Background The study investigates the diagnostic value of calretinin immunohistochemical staining (CIS) on rectal suction biopsies (RSB) in Hirschsprung's disease (HD). Methods A prospective study was conducted at Children's Hospital 2 in Ho Chi Minh City, Vietnam, from January through December 2015. Patients suspected of HD during this period underwent RSB and were followed in order to assess the accuracy of the diagnostic test with CIS compared with conventional histology (H&E). Results A total of 188 children with RSB were investigated. Median age was 7.1 (range 0.2–159) months with 65.4% boys. HD was confirmed in 80 (42.6%) children. There were 1 false positive and no false-negative cases. The sensitivity and specificity were 100% (80/80) and 99.1% (107/108) for CIS and 100% and 85.2% for H&E, respectively. Cohen's kappa coefficient was 0.9891 with a diagnostic accuracy of 99.5% for CIS, compared with 0.8303 and 91.5% for H&E, respectively. There were no serious complications related to the RSB. Conclusion RSB with CIS is a useful diagnostic method for HD, with easy interpretation and no need for cryostat. CIS has a high diagnostic accuracy and should be considered as the primary method for the diagnosis of HD by RSB. Level of evidence: Diagnostic Studies – Level I.

AB - Background The study investigates the diagnostic value of calretinin immunohistochemical staining (CIS) on rectal suction biopsies (RSB) in Hirschsprung's disease (HD). Methods A prospective study was conducted at Children's Hospital 2 in Ho Chi Minh City, Vietnam, from January through December 2015. Patients suspected of HD during this period underwent RSB and were followed in order to assess the accuracy of the diagnostic test with CIS compared with conventional histology (H&E). Results A total of 188 children with RSB were investigated. Median age was 7.1 (range 0.2–159) months with 65.4% boys. HD was confirmed in 80 (42.6%) children. There were 1 false positive and no false-negative cases. The sensitivity and specificity were 100% (80/80) and 99.1% (107/108) for CIS and 100% and 85.2% for H&E, respectively. Cohen's kappa coefficient was 0.9891 with a diagnostic accuracy of 99.5% for CIS, compared with 0.8303 and 91.5% for H&E, respectively. There were no serious complications related to the RSB. Conclusion RSB with CIS is a useful diagnostic method for HD, with easy interpretation and no need for cryostat. CIS has a high diagnostic accuracy and should be considered as the primary method for the diagnosis of HD by RSB. Level of evidence: Diagnostic Studies – Level I.

KW - Acetyl cholinesterase

KW - Calretinin immunochemistry

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KW - Hematoxylin & eosin

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