Developmental regulation of somatostatin gene expression in the brain is region specific

W. L. Lowe, A. E. Schaffner, Charles Roberts, D. LeRoith

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Developmental regulation of somatostatin (SRIF) gene expression was studied in five regions of rat brain and in rat stomach. Total RNA was isolated from hypothalamus, cortex, brainstem, cerebellum, and olfactory bulb, as well as stomach at eight stages of development from prenatal day 16 to postnatal day 82. Hybridization of a 32P-labeled rat SRIF cDNA probe to Northern blots of total RNA from the above tissues during development demonstrated a single hybridizing band approximately 670 base pairs in length. When SRIF mRNA levels from each stage of development were quantified and normalized by the amount of poly (A)+ RNA present at that stage of development, a unique pattern of SRIF gene expression was seen in each region. In brainstem and cerebellum, SRIF mRNA levels peaked early in development between prenatal day 21 and postnatal day 8 and then declined until postnatal day 82. Hypothalamus and cortex, on the other hand, showed a progressive increase during development with peak levels occurring between postnatal days 13 and 82. In contrast, stomach and olfactory bulb showed SRIF mRNA levels which were low during early development and which rose late in development (postnatal days 13 to 82). Marked differences in the amount of SRIF mRNA within each region were present as well. These data suggest that there is differential expression of the SRIF gene in different regions of the brain and in the stomach during development. Further study of this phenomenon may provide insight into the in vivo control of SRIF gene expression and the role of SRIF in the developing brain.

Original languageEnglish (US)
Pages (from-to)181-187
Number of pages7
JournalMolecular Endocrinology
Volume1
Issue number2
StatePublished - 1987
Externally publishedYes

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Developmental Gene Expression Regulation
Somatostatin
Stomach
Messenger RNA
Brain
Gene Expression
Olfactory Bulb
Cerebellum
Hypothalamus
Brain Stem
RNA
Base Pairing
Northern Blotting
Complementary DNA

ASJC Scopus subject areas

  • Molecular Biology
  • Endocrinology, Diabetes and Metabolism

Cite this

Developmental regulation of somatostatin gene expression in the brain is region specific. / Lowe, W. L.; Schaffner, A. E.; Roberts, Charles; LeRoith, D.

In: Molecular Endocrinology, Vol. 1, No. 2, 1987, p. 181-187.

Research output: Contribution to journalArticle

Lowe, W. L. ; Schaffner, A. E. ; Roberts, Charles ; LeRoith, D. / Developmental regulation of somatostatin gene expression in the brain is region specific. In: Molecular Endocrinology. 1987 ; Vol. 1, No. 2. pp. 181-187.
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