Developmental regulation of phospholipid methylation in rat brain synaptosomes

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10 Scopus citations

Abstract

Phospholipid methylation was studied in cortical synaptosomes prepared from 7 and 14 day and adult rat brain. Using varying concentrations of [3H] S-adenosylmethionine, Km and Vmax values were determined for the formation of [3H] phosphatidylmonomethylethanolamine (PME), [3H] phosphatidyl-dimethylethanolamine and [3H] phosphatidylcholine (PC). At 25°C, the Km values for the formation of all three products, significantly decreased with development. Increasing the temperature to 37°C increased the Km values in the 14 day and adult but not the 7 day preparation. The Vmax values at 25°C were highest at 7 and 14 days, depending on the product and then decreased in the adult. At 37°C, the Vmax values were highest in the 14 day preparation. The overall results are discussed in terms of the developmentally regulated decrease in both synaptic membrane PC and membrane fluidity.

Original languageEnglish (US)
Pages (from-to)1297-1303
Number of pages7
JournalLife Sciences
Volume30
Issue number15
DOIs
StatePublished - Apr 12 1982

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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