Developmental neurotoxicity of toluene: In vivo and in vitro effects on astroglial cells

Mark Burry, Marina Guizzetti, Jan Oberdoerster, Lucio G. Costa

Research output: Contribution to journalArticle

18 Scopus citations

Abstract

Toluene, an inexpensive and available industrial solvent, has become increasingly popular as a drug of abuse. Inhaling toluene leads to a feeling of euphoria and several reports have shown that children born to women who had abused toluene during pregnancy present a syndrome (toluene embryopathy or fetal solvent syndrome) that is characterized by CNS effects (e.g. microencephaly), growth retardation and facial dysmorphologies. The characteristics of the fetal solvent syndrome are very similar to those observed in the fetal alcohol syndrome. As exposure of rats to ethanol during the brain growth spurt has been shown to cause microencephaly and to affect glial cell proliferation and maturation, the present study examines the effects of toluene administration (250, 500 and 750 mg/kg) in neonatal rats from postnatal day 4 to 10. This treatment resulted in a significant decrease in both brain and body weights, and in a significant reduction of levels of glial fibrillary acidic protein, but not of neuron-specific enolase in rat brain. In vitro experiments demonstrate that pharmacologically relevant concentrations of toluene (250-1,000 μM) significantly inhibit proliferation of rat cortical astrocytes without causing overt cytotoxicity. These results indicate that toluene does not cause selective microencephaly; however, it affects brain weight, and appears to target developing astrocytes, possibly by inhibiting their proliferation.

Original languageEnglish (US)
Pages (from-to)14-19
Number of pages6
JournalDevelopmental Neuroscience
Volume25
Issue number1
DOIs
StatePublished - Aug 8 2003

Keywords

  • Developmental neurotoxicity
  • Fetal solvent syndrome
  • Glial cells
  • Toluene

ASJC Scopus subject areas

  • Neurology
  • Developmental Neuroscience

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