Development of Diubiquitin-Based FRET Probes to Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes

Paul P. Geurink, Bianca D.M. Van Tol, Duco Van Dalen, Paul J.G. Brundel, Tycho E.T. Mevissen, Jonathan Pruneda, Paul R. Elliott, Gabriëlle B.A. Van Tilburg, David Komander, Huib Ovaa

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

Deubiquitinating enzymes (DUBs) are proteases that fulfill crucial roles in the ubiquitin (Ub) system, by deconjugation of Ub from its targets and disassembly of polyUb chains. The specificity of a DUB towards one of the polyUb chain linkages largely determines the ultimate signaling function. We present a novel set of diubiquitin FRET probes, comprising all seven isopeptide linkages, for the absolute quantification of chain cleavage specificity of DUBs by means of Michaelis-Menten kinetics. Each probe is equipped with a FRET pair consisting of Rhodamine110 and tetramethylrhodamine to allow the fully synthetic preparation of the probes by SPPS and NCL. Our synthetic strategy includes the introduction of N,N′-Boc-protected 5-carboxyrhodamine as a convenient building block in peptide chemistry. We demonstrate the value of our probes by quantifying the linkage specificities of a panel of nine DUBs in a high-throughput manner.

Original languageEnglish (US)
Pages (from-to)816-820
Number of pages5
JournalChemBioChem
Volume17
Issue number9
DOIs
StatePublished - May 3 2016
Externally publishedYes

Fingerprint

Ubiquitin
Enzymes
Peptide Hydrolases
Peptides
Throughput
Kinetics
Deubiquitinating Enzymes
stable plasma protein solution
tetramethylrhodamine

Keywords

  • deubiquitinating enzymes
  • FRET
  • native chemical ligation
  • solid-phase synthesis
  • ubiquitin conjugates

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Molecular Biology
  • Organic Chemistry

Cite this

Geurink, P. P., Van Tol, B. D. M., Van Dalen, D., Brundel, P. J. G., Mevissen, T. E. T., Pruneda, J., ... Ovaa, H. (2016). Development of Diubiquitin-Based FRET Probes to Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes. ChemBioChem, 17(9), 816-820. https://doi.org/10.1002/cbic.201600017

Development of Diubiquitin-Based FRET Probes to Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes. / Geurink, Paul P.; Van Tol, Bianca D.M.; Van Dalen, Duco; Brundel, Paul J.G.; Mevissen, Tycho E.T.; Pruneda, Jonathan; Elliott, Paul R.; Van Tilburg, Gabriëlle B.A.; Komander, David; Ovaa, Huib.

In: ChemBioChem, Vol. 17, No. 9, 03.05.2016, p. 816-820.

Research output: Contribution to journalArticle

Geurink, PP, Van Tol, BDM, Van Dalen, D, Brundel, PJG, Mevissen, TET, Pruneda, J, Elliott, PR, Van Tilburg, GBA, Komander, D & Ovaa, H 2016, 'Development of Diubiquitin-Based FRET Probes to Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes', ChemBioChem, vol. 17, no. 9, pp. 816-820. https://doi.org/10.1002/cbic.201600017
Geurink, Paul P. ; Van Tol, Bianca D.M. ; Van Dalen, Duco ; Brundel, Paul J.G. ; Mevissen, Tycho E.T. ; Pruneda, Jonathan ; Elliott, Paul R. ; Van Tilburg, Gabriëlle B.A. ; Komander, David ; Ovaa, Huib. / Development of Diubiquitin-Based FRET Probes to Quantify Ubiquitin Linkage Specificity of Deubiquitinating Enzymes. In: ChemBioChem. 2016 ; Vol. 17, No. 9. pp. 816-820.
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