Development of a label-free imaging technique for the quantification of thrombus formation

Sandra M. Baker, Kevin G. Phillips, Owen McCarty

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

The characterization of platelet aggregation and thrombus formation typically requires the use of fluorescent labels followed by fluorescent confocal microscopy. However, fluorescent labels have been suspected to affect platelet function. We have developed a label-free imaging technique to characterize the volume and surface area coverage of platelet aggregates and thrombi formed under shear. Platelet aggregates were formed by perfusing anti-coagulated whole blood over fibrillar collagen. Thrombi were formed by perfusing recalcified whole blood over fibrillar collagen in the presence of coagulation. Platelet aggregates and thrombi volume and surface area coverage were quantified using a Hilbert transform differential interference contrast (DIC) microscopy technique (HT-DIC). Our data indicate that platelet aggregates and thrombi formed at a shear rate of 200 s-1 had similar volume and surface area coverage. At a shear rate of 1000 s-1, both the volume and surface area coverage of platelet aggregates significantly increased as compared to low shear conditions. In contrast, the volume of thrombi formed in the presence of coagulation appeared to remain the same at both low and high shear rates. Utilization of this HT-DIC imaging technique can allow for insights into the kinetics and mechanisms by which thrombi are formed under various shear conditions in a label-free manner.

Original languageEnglish (US)
Pages (from-to)488-492
Number of pages5
JournalCellular and Molecular Bioengineering
Volume5
Issue number4
DOIs
StatePublished - Dec 2012

Fingerprint

Platelets
Quantification
Labels
Thrombosis
Imaging
Blood Platelets
Imaging techniques
Surface area
Coverage
Fibrillar Collagens
Shear deformation
Collagen
Coagulation
Interference
Blood
Interference Microscopy
Confocal Microscopy
Hilbert Transform
Confocal microscopy
Platelet Aggregation

Keywords

  • Blood
  • Coagulation
  • Microscopy

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Modeling and Simulation

Cite this

Development of a label-free imaging technique for the quantification of thrombus formation. / Baker, Sandra M.; Phillips, Kevin G.; McCarty, Owen.

In: Cellular and Molecular Bioengineering, Vol. 5, No. 4, 12.2012, p. 488-492.

Research output: Contribution to journalArticle

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