Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta)

Cynthia M Lester McCully, John Bacher, Rhonda MacAllister, Emilie A. Steffen-Smith, Kadharbatcha Saleem, Marvin L. Thomas, Rafael Cruz, Katherine E. Warren

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Rapid, serial, and humane collection of cerebrospinal fluid (CSF) in nonhuman primates (NHP) is an essential element of numerous research studies and is currently accomplished via two different models. The CSF reservoir model (FR) combines a catheter in the 4th ventricle with a flexible silastic reservoir to permit circulating CSF flow. The CSF lateral port model (LP) consists of a lateral ventricular catheter and an IV port that provides static access to CSF and volume restrictions on sample collection. The FR model is associated with an intensive, prolonged recovery and frequent postsurgical hydrocephalus and nonpatency, whereas the LP model is associated with an easier recovery. To maximize the advantages of both systems, we developed the CSF lateral reservoir model (LR), which combines the beneficial features of the 2 previous models but avoids their limitations by using a reservoir for circulating CSF flow combined with catheter placement in the lateral ventricle. Nine adult male rhesus monkeys were utilized in this study. Pre-surgical MRI was performed to determine the coordinates of the lateral ventricle and location of choroid plexus (CP). The coordinates were determined to avoid the CP and major blood vessels. The predetermined coordinates were 100% accurate, according to MRI validation. The LR system functioned successfully in 67% of cases for 221 d, and 44% remain functional at 426 to 510 d postoperatively. Compared with established models, our LR model markedly reduced postoperative complications and recovery time. Development of the LR model was successful in rhesus macaques and is a useful alternative to the FR and LP methods of CSF collection from nonhuman primates.

Original languageEnglish (US)
Pages (from-to)77-82
Number of pages6
JournalComparative Medicine
Volume65
Issue number1
StatePublished - Feb 1 2015
Externally publishedYes

Fingerprint

Cerebrospinal fluid
cerebrospinal fluid
Macaca mulatta
Cerebrospinal Fluid
Choroid Plexus
Lateral Ventricles
Primates
Catheters
catheters
choroid plexus
Fourth Ventricle
Vascular Access Devices
Hydrocephalus
Recovery
Magnetic resonance imaging
Flow of fluids
Blood Vessels
hydrocephalus
postoperative complications
Blood vessels

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • veterinary(all)

Cite this

McCully, C. M. L., Bacher, J., MacAllister, R., Steffen-Smith, E. A., Saleem, K., Thomas, M. L., ... Warren, K. E. (2015). Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta). Comparative Medicine, 65(1), 77-82.

Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta). / McCully, Cynthia M Lester; Bacher, John; MacAllister, Rhonda; Steffen-Smith, Emilie A.; Saleem, Kadharbatcha; Thomas, Marvin L.; Cruz, Rafael; Warren, Katherine E.

In: Comparative Medicine, Vol. 65, No. 1, 01.02.2015, p. 77-82.

Research output: Contribution to journalArticle

McCully, CML, Bacher, J, MacAllister, R, Steffen-Smith, EA, Saleem, K, Thomas, ML, Cruz, R & Warren, KE 2015, 'Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta)', Comparative Medicine, vol. 65, no. 1, pp. 77-82.
McCully CML, Bacher J, MacAllister R, Steffen-Smith EA, Saleem K, Thomas ML et al. Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta). Comparative Medicine. 2015 Feb 1;65(1):77-82.
McCully, Cynthia M Lester ; Bacher, John ; MacAllister, Rhonda ; Steffen-Smith, Emilie A. ; Saleem, Kadharbatcha ; Thomas, Marvin L. ; Cruz, Rafael ; Warren, Katherine E. / Development of a cerebrospinal fluid lateral reservoir model in rhesus monkeys (Macaca mulatta). In: Comparative Medicine. 2015 ; Vol. 65, No. 1. pp. 77-82.
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