Determine scattering coefficient and anisotropy of scattering of murine tissues using reflectance-mode confocal microscopy

Ravikant Samatham, Steven Jacques

Research output: Chapter in Book/Report/Conference proceedingConference contribution

3 Citations (Scopus)

Abstract

Different techniques have been developed to determine the optical properties of turbid media, which include collimated transmission, diffuse reflectance, adding-doubling and goniometry. While goniometry can be used to determine the anisotropy of scattering (g), other techniques are used to measure the absorption coefficient and reduced scattering coefficient (μs(1-g)). But separating scattering coefficient (μs) and anisotropy of scattering from reduced scattering coefficient has been tricky. We developed an algorithm to determine anisotropy of scattering from the depth dependent decay of reflectance-mode confocal scanning laser microscopy (rCSLM) data. This report presents the testing of the algorithm on tissue phantoms with different anisotropies (g = 0.127 to 0.868, at 488 nm wavelength). Tissue phantoms were made from polystyrene microspheres (6 sizes 0.1-0.5 μm dia.) dispersed in both aqueous solutions and agarose gels. Three dimensional images were captured. The rCSLM-signal followed an exponential decay as a function of depth of the focal volume, R(z)ρexp(-μz) where ρ (dimensionless, ρ = 1 for a mirror) is the local reflectivity and μ [cm-1] is the exponential decay constant. The theory was developed to uniquely map the experimentally determined μ and ρ into the optical scattering properties μs and g. The values of μs and g depend on the composition and microstructure of tissues, and allow characterization of a tissue.

Original languageEnglish (US)
Title of host publicationProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume8592
DOIs
StatePublished - 2013
EventBiomedical Applications of Light Scattering VII - San Francisco, CA, United States
Duration: Feb 2 2013Feb 4 2013

Other

OtherBiomedical Applications of Light Scattering VII
CountryUnited States
CitySan Francisco, CA
Period2/2/132/4/13

Fingerprint

Confocal microscopy
Anisotropy
scattering coefficients
Laser modes
Confocal Microscopy
Scattering
Tissue
laser microscopy
microscopy
reflectance
anisotropy
scattering
decay
scanning
Three-Dimensional Imaging
Polystyrenes
Microspheres
Microscopic examination
Sepharose
polystyrene

Keywords

  • anisotropy of scattering
  • coe cient of scattering
  • murine tissue
  • optical scattering properties
  • reectance-mode confocal scanning laser microscope

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

Cite this

Samatham, R., & Jacques, S. (2013). Determine scattering coefficient and anisotropy of scattering of murine tissues using reflectance-mode confocal microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE (Vol. 8592). [85920U] https://doi.org/10.1117/12.2005072

Determine scattering coefficient and anisotropy of scattering of murine tissues using reflectance-mode confocal microscopy. / Samatham, Ravikant; Jacques, Steven.

Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8592 2013. 85920U.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Samatham, R & Jacques, S 2013, Determine scattering coefficient and anisotropy of scattering of murine tissues using reflectance-mode confocal microscopy. in Progress in Biomedical Optics and Imaging - Proceedings of SPIE. vol. 8592, 85920U, Biomedical Applications of Light Scattering VII, San Francisco, CA, United States, 2/2/13. https://doi.org/10.1117/12.2005072
Samatham R, Jacques S. Determine scattering coefficient and anisotropy of scattering of murine tissues using reflectance-mode confocal microscopy. In Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8592. 2013. 85920U https://doi.org/10.1117/12.2005072
Samatham, Ravikant ; Jacques, Steven. / Determine scattering coefficient and anisotropy of scattering of murine tissues using reflectance-mode confocal microscopy. Progress in Biomedical Optics and Imaging - Proceedings of SPIE. Vol. 8592 2013.
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