Determination of serotonin and 5‐hydroxyindoleacetic acid in guinea pig and human prostate using HPLC

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The finding of significant numbers of endocrine‐paracrine (EP) cells in the prostate glands of guinea pigs and man suggests that these cells may be important in the regulation or modulation of prostatic function. Serotonin is a biogenic amine common to most prostatic EP cells. In order to extend current knowledge relating to these cells, an assay was developed using high‐performance liquid chromatography to quantitate serotonin and its metabolite 5‐hydroxyindoleacetic acid (5‐HIAA) in guinea pig and human prostatic tissue extracts. Levels of serotonin and 5‐HIAA in the guinea pig whole‐gland preparation were 105.4 ± 70.6 ng/g and 48.4 ± 95.7 ng/g, respectively. Normal human prostatic tissue contained 1423.9 ± 750.8 ng/g serotonin and 66.7 ± 92.8 ng/g 5‐HIAA. Recoveries ranged between 60 and 100%. The detection limits were 24 pg/injection for serotonin and 12 pg/injection for 5‐HIAA. This assay provides an expeditious, specific and highly sensitive method for the simultaneous determination of monoamines in guinea pig and human prostatic tissue.

Original languageEnglish (US)
Pages (from-to)353-360
Number of pages8
JournalThe Prostate
Issue number4
StatePublished - 1987



  • 5‐HIAA
  • electrochemical detection
  • monoamines
  • neuroendocrine cells

ASJC Scopus subject areas

  • Oncology
  • Urology

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