Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC)

Ana Konvalinka, Joyce Zhou, Apostolos Dimitromanolakis, Andrei P. Drabovich, Fei Fang, Susan Gurley, Thomas Coffman, Rohan John, Shao Ling Zhang, Eleftherios P. Diamandis, James W. Scholey

Research output: Contribution to journalArticle

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Abstract

Background: Specific markers of kidney angiotensin-II (AngII) activity are needed. Results: Eighteen AngII-regulated proteins were discovered and confirmed by proteomics in human kidney cells, whereas heme oxygenase-1 was validated in a mouse. Conclusion: Heme oxygenase-1 and other AngII-regulated proteins represent novel markers of AngII activity. Significance: AngII-regulated proteins may represent kidney-specific AngII activity markers in patients and in experimental models. Angiotensin II (AngII), the major effector of the renin-angiotensin system, mediates kidney disease progression by signaling through the AT-1 receptor (AT-1R), but there are no specific measures of renal AngII activity. Accordingly, we sought to define an AngII-regulated proteome in primary human proximal tubular cells (PTEC) to identify potential AngII activity markers in the kidney. We utilized stable isotope labeling with amino acids (SILAC) in PTECs to compare proteomes of AngIItreated and control cells. Of the 4618 quantified proteins, 83 were differentially regulated. SILAC ratios for 18 candidates were confirmed by a different mass spectrometry technique called selected reaction monitoring. Both SILAC and selected reaction monitoring revealed heme oxygenase-1 (HO-1) as the most significantly up-regulated protein in response to AngII stimulation. AngII-dependent regulation of the HO-1 gene and protein was further verified in PTECs. To extend these in vitro observations, we overlaid a network of significantly enriched gene ontology terms from our AngII-regulated proteins with a dataset of differentially expressed kidney genes from AngII-treated wild type mice and AT-1R knock-out mice. Five gene ontology terms were enriched in both datasets and included HO-1. Furthermore, HO-1 kidney expression and urinary excretion were reduced in AngIItreated mice with PTEC-specific AT-1R deletion compared with AngII-treated wild-type mice, thus confirming AT-1R-mediated regulation of HO-1. Our in vitro approach identified novel molecular markers of AngII activity, and the animal studies demonstrated that these markers are relevant in vivo. These interesting proteins hold promise as specific markers of renal AngII activity in patients and in experimental models.

Original languageEnglish (US)
Pages (from-to)24834-24847
Number of pages14
JournalJournal of Biological Chemistry
Volume288
Issue number34
DOIs
StatePublished - Aug 23 2013
Externally publishedYes

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Isotope Labeling
Proteome
Cell culture
Isotopes
Angiotensin II
Labeling
Cell Culture Techniques
Kidney
Amino Acids
Heme Oxygenase-1
Proteins
Gene Ontology
Genes
Ontology
Theoretical Models

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC). / Konvalinka, Ana; Zhou, Joyce; Dimitromanolakis, Apostolos; Drabovich, Andrei P.; Fang, Fei; Gurley, Susan; Coffman, Thomas; John, Rohan; Zhang, Shao Ling; Diamandis, Eleftherios P.; Scholey, James W.

In: Journal of Biological Chemistry, Vol. 288, No. 34, 23.08.2013, p. 24834-24847.

Research output: Contribution to journalArticle

Konvalinka, A, Zhou, J, Dimitromanolakis, A, Drabovich, AP, Fang, F, Gurley, S, Coffman, T, John, R, Zhang, SL, Diamandis, EP & Scholey, JW 2013, 'Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC)', Journal of Biological Chemistry, vol. 288, no. 34, pp. 24834-24847. https://doi.org/10.1074/jbc.M113.485326
Konvalinka, Ana ; Zhou, Joyce ; Dimitromanolakis, Apostolos ; Drabovich, Andrei P. ; Fang, Fei ; Gurley, Susan ; Coffman, Thomas ; John, Rohan ; Zhang, Shao Ling ; Diamandis, Eleftherios P. ; Scholey, James W. / Determination of an angiotensin II-regulated proteome in primary human kidney cells by stable isotope labeling of amino acids in cell culture (SILAC). In: Journal of Biological Chemistry. 2013 ; Vol. 288, No. 34. pp. 24834-24847.
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AU - Zhou, Joyce

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AU - Drabovich, Andrei P.

AU - Fang, Fei

AU - Gurley, Susan

AU - Coffman, Thomas

AU - John, Rohan

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AU - Scholey, James W.

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