Detection of Merkel cell virus and correlation with histologic presence of Merkel cell carcinoma in sentinel lymph nodes

Myriam Loyo, J. Schussel, E. Colantuoni, J. Califano, M. Brait, S. Kang, W. M. Koch, D. Sidransky, W. H. Westra, J. M. Taube

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background: Adjuvant treatment can dramatically improve the survival of patients with metastatic Merkel cell carcinoma (MCC), making early, accurate detection of nodal disease critical. The purpose of this study was to correlate Merkel cell virus (MCV) detection with histopathologic disease in sentinel lymph nodes (SLNs) of MCC. Methods: Merkel cell carcinoma cases with SLN (n=25) were compared with negative controls (n=27). Viral load was obtained by quantitative polymerase chain reaction (PCR) for regions VP1 and LT3 of MCV. Histopathologic disease and viral load were correlated. Results: Merkel cell virus was detected in 16 out of 17 (94%) of primary MCC (mean viral load (MVL)1.44 copies per genome). Viral load in the negative controls was

Original languageEnglish (US)
Pages (from-to)1314-1319
Number of pages6
JournalBritish Journal of Cancer
Volume106
Issue number7
DOIs
StatePublished - Mar 27 2012
Externally publishedYes

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Merkel Cells
Merkel Cell Carcinoma
Viral Load
Viruses
Genome
Polymerase Chain Reaction
Survival
Sentinel Lymph Node

Keywords

  • Merkel cell carcinoma
  • Merkel cell polyomavirus
  • RT-PCR
  • sentinel lymph node
  • tumour staging

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Detection of Merkel cell virus and correlation with histologic presence of Merkel cell carcinoma in sentinel lymph nodes. / Loyo, Myriam; Schussel, J.; Colantuoni, E.; Califano, J.; Brait, M.; Kang, S.; Koch, W. M.; Sidransky, D.; Westra, W. H.; Taube, J. M.

In: British Journal of Cancer, Vol. 106, No. 7, 27.03.2012, p. 1314-1319.

Research output: Contribution to journalArticle

Loyo, M, Schussel, J, Colantuoni, E, Califano, J, Brait, M, Kang, S, Koch, WM, Sidransky, D, Westra, WH & Taube, JM 2012, 'Detection of Merkel cell virus and correlation with histologic presence of Merkel cell carcinoma in sentinel lymph nodes', British Journal of Cancer, vol. 106, no. 7, pp. 1314-1319. https://doi.org/10.1038/bjc.2012.73
Loyo, Myriam ; Schussel, J. ; Colantuoni, E. ; Califano, J. ; Brait, M. ; Kang, S. ; Koch, W. M. ; Sidransky, D. ; Westra, W. H. ; Taube, J. M. / Detection of Merkel cell virus and correlation with histologic presence of Merkel cell carcinoma in sentinel lymph nodes. In: British Journal of Cancer. 2012 ; Vol. 106, No. 7. pp. 1314-1319.
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AU - Brait, M.

AU - Kang, S.

AU - Koch, W. M.

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AU - Westra, W. H.

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