Abstract
Co-immunoprecipitation is an invaluable technique in evaluating native protein-protein interactions in vitro and in vivo. However, it can be difficult to detect interactions of a very transient nature, particularly interactions with phosphatases and kinases. The evaluation of the phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe two protocols: one for the co-immunoprecipitation of endogenous c-Myc to detect protein-protein interactions and second, for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status.
Original language | English (US) |
---|---|
Title of host publication | Methods in Molecular Biology |
Pages | 65-76 |
Number of pages | 12 |
Volume | 1012 |
DOIs | |
State | Published - 2013 |
ASJC Scopus subject areas
- Molecular Biology
- Genetics