Detection of c-Myc protein-protein interactions and phosphorylation status by immunoprecipitation

Colin J. Daniel, Xiaoli Zhang, Rosalie C. Sears

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Co-immunoprecipitation is an invaluable technique in evaluating native protein-protein interactions in vitro and in vivo. However, it can be difficult to detect interactions of a very transient nature, particularly interactions with phosphatases and kinases. The evaluation of the phosphorylation status of c-Myc can also be challenging with the current commercially available phosphorylation sensitive antibodies. Here, we describe two protocols: one for the co-immunoprecipitation of endogenous c-Myc to detect protein-protein interactions and second, for the immunoprecipitation of endogenous c-Myc to probe for phosphorylation status.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
Pages65-76
Number of pages12
Volume1012
DOIs
StatePublished - 2013

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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